Hypoxia-induced metastasis model in embryonic zebrafish

Hypoxia facilitates tumor invasion and metastasis by promoting neovascularization and co-option of tumor cells in the peritumoral vasculature, leading to dissemination of tumor cells into the circulation. However, until recently, animal models and imaging technology did not enable monitoring of the early events of tumor cell invasion and dissemination in living animals. We recently developed a zebrafish metastasis model to dissect the detailed events of hypoxia-induced tumor cell invasion and metastasis in association with angiogenesis at the single-cell level. In this model, fluorescent DiI-labeled human or mouse tumor cells are implanted into the perivitelline cavity of 48-h-old zebrafish embryos, which are subsequently placed in hypoxic water for 3 d. Tumor cell invasion, metastasis and pathological angiogenesis are detected under fluorescent microscopy in the living fish. The average experimental time for this model is 7 d. Our protocol offers a remarkable opportunity to study molecular mechanisms of hypoxia-induced cancer metastasis.

Jensen, Lasse DKarolinska Institutet(Swepub:liu)larje86 (author)
Cao, ZiquanLinköpings universitet,Avdelningen för kardiovaskulär medicin,Hälsouniversitetet(Swepub:liu)ziqca08 (author)
Hosaka, KayokoKarolinska Institutet (author)
Länne, TosteÖstergötlands Läns Landsting,Linköpings universitet,Fysiologi,Hälsouniversitetet,Thorax-kärlkliniken(Swepub:liu)tosla34 (author)
Wahlberg, EricÖstergötlands Läns Landsting,Linköpings universitet,Kärlkirurgi,Hälsouniversitetet,Thorax-kärlkliniken(Swepub:liu)eriwa73 (author)
Fleng Steffensen, JohnMarine Biological Laboratory, Biological Institute, University of Copenhagen, Helsingor, Denmark (author)
Cao, YihaiKarolinska Institutet,Linköpings universitet,Avdelningen för kardiovaskulär medicin,Hälsouniversitetet,Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden(Swepub:liu)yihca64 (author)
Karolinska InstitutetAvdelningen för kardiovaskulär medicin (creator_code:org_t)

In:Nature Protocols: Nature Publishing Group5:12, s. 1911-19181754-21891750-2799

By the author/editor: Rouhi, Pegah; Jensen, Lasse D; Cao, Ziquan; Hosaka, Kayoko; Länne, Toste; Wahlberg, Eric; show more...; Fleng Steffensen ...; Cao, Yihai; show less...

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