Sökning: WFRF:(TURNER APF)
> (2005-2009) >
Screen-printed bios...
Screen-printed biosensor for allergens
-
- Sarkar, P (författare)
- University Calcutta, Department Polymer Science and Technology, Calcutta 700009, W Bengal, India; University Durham, Department Chemistry, Durham DH1 3LE, England; Cranfield University, Institute Biosci and Technology, Silsoe MK45 4DT, Beds, England;
-
- Ghosh, D (författare)
- University Calcutta, Department Polymer Science and Technology, Calcutta 700009, W Bengal, India; University Durham, Department Chemistry, Durham DH1 3LE, England; Cranfield University, Institute Biosci and Technology, Silsoe MK45 4DT, Beds, England;
-
- Bhattacharya, D (författare)
- University Calcutta, Department Polymer Science and Technology, Calcutta 700009, W Bengal, India; University Durham, Department Chemistry, Durham DH1 3LE, England; Cranfield University, Institute Biosci and Technology, Silsoe MK45 4DT, Beds, England;
-
visa fler...
-
- Kataky, R (författare)
- University Calcutta, Department Polymer Science and Technology, Calcutta 700009, W Bengal, India; University Durham, Department Chemistry, Durham DH1 3LE, England; Cranfield University, Institute Biosci and Technology, Silsoe MK45 4DT, Beds, England;
-
- Setford, SJ (författare)
- University Calcutta, Department Polymer Science and Technology, Calcutta 700009, W Bengal, India; University Durham, Department Chemistry, Durham DH1 3LE, England; Cranfield University, Institute Biosci and Technology, Silsoe MK45 4DT, Beds, England;
-
- White, SF (författare)
- University Calcutta, Department Polymer Science and Technology, Calcutta 700009, W Bengal, India; University Durham, Department Chemistry, Durham DH1 3LE, England; Cranfield University, Institute Biosci and Technology, Silsoe MK45 4DT, Beds, England;
-
- Turner, APF (författare)
- Cranfield University, UK
-
visa färre...
-
(creator_code:org_t)
- 2005
- 2005
- Engelska.
-
Ingår i: Journal of chemical technology and biotechnology (1986). - : John Wiley andamp;amp; Sons, Ltd. - 0268-2575 .- 1097-4660. ; 80:12, s. 1389-1394
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Allergen levels in indoor environments, leading to many diseases, eg asthma, rhinitis and conjunctivitis, affect a large and increasing fraction of the population. A quite effective and inexpensive method of a rough but very rapid overall assessment of total allergen level in the environment has been developed. The method involved estimation of protein in allergen extracts by screen-printed electrodes using two different techniques. The biosensor comprised a rhodinised carbon working electrode, a silver/silver chloride reference electrode and a carbon counter electrode. In the first method the enzyme protease reacted with allergen protein to release amino acid, which produced hydrogen peroxide in the presence of amino acid oxidase. This was detected amperometrically. The second method used potassium bromide as electrolyte and the electrode was subjected to dual potential. Bromine, released due to electrolysis at higher potential, was consumed by the allergen protein at lower potential. In the first method, a unique technique was used to microencapsulate the enzyme protease and immobilise it on the surface of the electrode by in-situ polymerisation to avoid contact with the amino acid oxidase. A total of seven allergens were tested and the results gave a good correlation with the standard protein measurement method. Environmental specimens from indoors, schools and workplaces can be evaluated for the aeroallergens produced by dust mites, animal hairs, cockroach debris, pollens, etc as a means of determining the exposure risk. (c) 2005 Society of Chemical Industry.
Nyckelord
- allergen; enzyme; biosensor; screen-printed electrode; amperometric
- TECHNOLOGY
- TEKNIKVETENSKAP
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas