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Screen-printed ampe...
Screen-printed amperometric biosensors for the rapid measurement of L- and D-amino acids
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- Sarkar, P (author)
- Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;
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- Tothill, IE (author)
- Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;
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- Setford, SJ (author)
- Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;
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- Turner, APF (author)
- Cranfield University, UK
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(creator_code:org_t)
- Royal Society of Chemistry, 1999
- 1999
- English.
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In: The Analyst. - : Royal Society of Chemistry. - 0003-2654 .- 1364-5528. ; 124:6, s. 865-870
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
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- Screen-printed three-electrode amperometric sensors incorporating L- and/or D-amino acid oxidase for the general purpose measurement of L- or D-amino acids is described. The working electrode incorporates rhodinised carbon, to facilitate hydrogen peroxide oxidation at a decreased operating potential, and immobilised enzyme. The devices responded to all 20 common L-amino acids and all of the D-amino acids examined, the exceptions being L- and D-proline. Linear response profiles were observed for L-leucine, L-glycine and L-phenylalanine with limits of detection of 0.47, 0.15 and 0.20 mM respectively. The devices were reproducible and exhibited stability over a 56 d test period. The biosensor compares favourably with a standard photometric amino acid test and was used to monitor milk ageing effects, The assay is cheap, simple to perform and rapid, requiring only buffer-electrolyte and a small sample volume.
Keyword
- TECHNOLOGY
- TEKNIKVETENSKAP
Publication and Content Type
- ref (subject category)
- art (subject category)
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