The synthesis and screening of a combinatorial peptide library for affinity ligands for glycosylated haemoglobin

Chen, BCranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; (author)

This paper reports the synthesis and screening of a combinatorial peptide library for new affinity ligands for glycosylated haemoglobin (HbA(1c)), which is an important indicator of diabetes control. The new ligands are suitable for large-scale synthesis and overcome the disadvantages of antibodies (unstable and expensive to produce etc.), while remaining as efficient as antibodies in binding to the analyte. The library consisted of 262 144 hexapeptides synthesised using the one-bead-one-compound technique. The hexapeptides attached onto beads were screened with glycosylated haemoglobin HbA(1c). The structures of the peptides exhibiting high affinity were characterised by Edman microsequencing. Computer modelling simulation of one of the lead sequences has shown that this class of ligand has a high affinity and specificity for glycosylated haemoglobin. (C) 1998 Elsevier Science S.A. All rights reserved.

affinity ligand; combinatorial peptide library; computer modelling simulation; diabetes; glycated proteins; glycosylated haemoglobin; one-bead one-compound
TECHNOLOGY
TEKNIKVETENSKAP

Bestetti, GCranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; (author)
Day, RMCranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; (author)
Turner, APFCranfield University, UK(Swepub:liu)anttu13 (author)
Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;Cranfield University, UK (creator_code:org_t)

In:Biosensors & bioelectronics: Elsevier Science B.V., Amsterdam.13:08-jul, s. 779-7850956-56631873-4235

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