Search: L773:0956 5663 OR L773:1873 4235
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The synthesis and s...
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Chen, BCranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;
(author)
The synthesis and screening of a combinatorial peptide library for affinity ligands for glycosylated haemoglobin
- Article/chapterEnglish1998
Publisher, publication year, extent ...
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Elsevier Science B.V., Amsterdam.1998
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:liu-65248
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-65248URI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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This paper reports the synthesis and screening of a combinatorial peptide library for new affinity ligands for glycosylated haemoglobin (HbA(1c)), which is an important indicator of diabetes control. The new ligands are suitable for large-scale synthesis and overcome the disadvantages of antibodies (unstable and expensive to produce etc.), while remaining as efficient as antibodies in binding to the analyte. The library consisted of 262 144 hexapeptides synthesised using the one-bead-one-compound technique. The hexapeptides attached onto beads were screened with glycosylated haemoglobin HbA(1c). The structures of the peptides exhibiting high affinity were characterised by Edman microsequencing. Computer modelling simulation of one of the lead sequences has shown that this class of ligand has a high affinity and specificity for glycosylated haemoglobin. (C) 1998 Elsevier Science S.A. All rights reserved.
Subject headings and genre
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affinity ligand; combinatorial peptide library; computer modelling simulation; diabetes; glycated proteins; glycosylated haemoglobin; one-bead one-compound
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TECHNOLOGY
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TEKNIKVETENSKAP
Added entries (persons, corporate bodies, meetings, titles ...)
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Bestetti, GCranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;
(author)
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Day, RMCranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;
(author)
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Turner, APFCranfield University, UK(Swepub:liu)anttu13
(author)
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Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;Cranfield University, UK
(creator_code:org_t)
Related titles
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In:Biosensors & bioelectronics: Elsevier Science B.V., Amsterdam.13:08-jul, s. 779-7850956-56631873-4235
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