ORGANIC-PHASE ENZYME ELECTRODES FOR THE DETERMINATION OF HYDROGEN-PEROXIDE AND PHENOL

• An amperometric horseradish peroxidase electrode is described for the determination of hydrogen peroxide in organic solvents. The enzyme was co-adsorbed with an electron mediator, hexacyanoferrate(II), to the surface of a graphite foil electrode making reagentless measurement possible. The electrochemical reduction of the enzymatically oxidized mediator was utilized as the analytical signal. The electrode can be operated in dioxane, chloroform and chlorobenzene, the presence of a small quantity of aqueous buffer being essential for activity. On this basis a small, probe-type sensor has been assembled the response of which is linearly related to hydrogen peroxide concentration between 0.05 and 1 mM. A tyrosinase sensor has been constructed by combining a Clark-type oxygen electrode with a membrane bearing adsorbed enzyme. The sensor is capable of measuring between 0.1 and 5 mM phenol in chloroform saturated with aqueous buffer.

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