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Effects of the Gona...
Effects of the Gonadotropin-Releasing Hormone Antagonist Ganirelix on Normal Micturition and Prostaglandin E-2-Induced Detrusor Overactivity in Conscious Female Rats
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- Russo, Andrea (författare)
- San Raffaele University
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- Castiglione, Fabio (författare)
- San Raffaele University
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- Salonia, Andrea (författare)
- San Raffaele University
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- Benigni, Fabio (författare)
- San Raffaele University
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- Rigatti, Patrizio (författare)
- San Raffaele University
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- Montorsi, Francesco (författare)
- San Raffaele University
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- Andersson, Karl-Erik (författare)
- Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine,Wake Forest Institute for Regenerative Medicine
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- Hedlund, Petter (författare)
- Lund University,Lunds universitet,Östergötlands Läns Landsting,Linköpings universitet,Klinisk farmakologi,Hälsouniversitetet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine
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(creator_code:org_t)
- ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, 2011
- 2011
- Engelska.
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Ingår i: EUROPEAN UROLOGY. - : ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS. - 0302-2838 .- 1873-7560. ; 59:5, s. 868-874
- Relaterad länk:
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http://www.ncbi.nlm....
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http://dx.doi.org/10...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- Background: Gonadotropin-releasing hormone (GnRH) antagonists have been reported to have beneficial effects on lower urinary tract symptoms in patients with benign prostatic hyperplasia. Objective: Our aim was to investigate the effects of ganirelix, a GnRH receptor antagonist, on bladder function and detrusor overactivity (DO) in female rats. Design, setting, and participants: Female Sprague-Dawley rats received 2 wk of daily systemic (0.1 mg/kg) or acute intravesical administration (IVES; 0.14 mg/l or 1.4 mg/l) ganirelix or vehicle (controls). Measurements: Assessments were obtained using cystometry in awake rats, organ bath studies, enzyme-linked immunosorbent assay, and western blot (WB). Results and limitations: Luteinising hormone levels were lower in rats treated systemically with ganirelix than in controls. No differences were observed in body or bladder weights. Micturition interval (MI), micturition volume (MV), residual volume, and bladder capacity (BC) were similar in both groups at baseline. No differences in urodynamic pressure parameters were observed between groups at baseline. Intravesical prostaglandin E-2 reduced MI, MV, and BC, and it increased basal pressure (BP), threshold pressure (TP), flow pressure (FP), and maximum pressure (MP) in all rats. MI, MV, and BC were reduced by 43% +/- 4%, 50% +/- 4%, and 43% +/- 4% (controls) versus 22% +/- 3%, 23% +/- 3%, and 21% +/- 3% (ganirelix-treated rats; p andlt; 0.001). TP and FP increased by 38% +/- 8% and 30% +/- 4% (controls) versus 16% +/- 7% and 16% +/- 5% (ganirelix; p andlt; 0.05). The maximal force of contractions for carbachol was larger in detrusor from ganirelix-treated rats (231% vs 177% of 60 mM K+-induced contractions). At 0.14 mg/l, but not 0.14 mg/l, IVES ganirelix increased MI, MV, and BC and decreased BP, TP, FP, and MP. In vitro, ganirelix had no effect on detrusor function. The gonadotropin-releasing hormone receptor was expressed (by WB) in the bladder mucosa. Conclusions: Systemic treatment with ganirelix counteracted experimental DO in female rats. Because bladder preparations from these rats exhibited larger contractions to carbachol and because intravesical ganirelix affected both micturition intervals and urodynamic pressure profiles, a peripheral site of action of ganirelix in the urinary bladder cannot be excluded.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Urologi och njurmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Urology and Nephrology (hsv//eng)
Nyckelord
- GnRH
- Receptor
- Mucosa
- Western blot
- Antagonist
- Systemic
- Intravesical
- Cystometry
- MEDICINE
- MEDICIN
- Receptor
- GnRH
- Mucosa
- Western blot
- Antagonist
- Systemic
- Intravesical
- Cystometry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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