Time-Lapse In Vivo Imaging of Corneal Angiogenesis: The Role of Inflammatory Cells in Capillary Sprouting

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: id:"swepub:oai:DiVA.org:liu-69178" > Time-Lapse In Vivo ...

1 av 1
Föregående post
Nästa post
Till träfflistan

Bourghardt Peebo, BeatriceÖstergötlands Läns Landsting,Linköpings universitet,Oftalmiatrik,Hälsouniversitetet,Ögonkliniken US/LiM (författare)

Time-Lapse In Vivo Imaging of Corneal Angiogenesis: The Role of Inflammatory Cells in Capillary Sprouting

Artikel/kapitelEngelska2011

Förlag, utgivningsår, omfång ...

Research in Vision and Opthalmology,2011
electronicrdacarrier

Nummerbeteckningar

LIBRIS-ID:oai:DiVA.org:liu-69178
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-69178URI
https://doi.org/10.1167/iovs.10-6101DOI

Kompletterande språkuppgifter

Språk:engelska
Sammanfattning på:engelska

Ingår i deldatabas

SwePubSwePub

Klassifikation

Ämneskategori:ref swepub-contenttype
Ämneskategori:art swepub-publicationtype

Anmärkningar

Original Publication: Beatrice Bourghardt Peebo, Per Fagerholm, Catharina Traneus-Rockert and Neil Lagali, Time-Lapse In Vivo Imaging of Corneal Angiogenesis: The Role of Inflammatory Cells in Capillary Sprouting, 2011, INVESTIGATIVE OPHTHALMOLOGY and VISUAL SCIENCE, (52), 6, 3060-3068. http://dx.doi.org/10.1167/iovs.10-6101 Copyright: Research in Vision and Opthalmology http://www.arvo.org/
PURPOSE. To elucidate the temporal sequence of events leading to new capillary sprouting in inflammatory corneal angiogenesis. METHODS. Angiogenesis was induced by corneal suture placement in Wistar rats. The inflamed region was examined by time-lapse in vivo confocal microscopy for up to 7 days. At 6 and 12 hours and 1, 2, 4, and 7 days, corneas were excised for flat mount immunofluorescence with primary antibodies for CD31, CD34, CD45, CD11b, CD11c, Ki-M2R, NG2, and alpha-SMA. From days 0 to 4, the in vivo extravasation and expansion characteristics of single limbal vessels were quantified. RESULTS. Starting hours after induction and peaking at day 1, CD45(+)CD11b(+) myeloid cells extravasated from limbal vessels and formed endothelium-free tunnels within the stroma en route to the inflammatory stimulus. Limbal vessel diameter tripled on days 2 to 3 as vascular buds emerged and transformed into perfused capillary sprouts less than 1 day later. A subset of spindle-shaped CD11b(+) myeloid-lineage cells, but not dendritic cells or mature macrophages, appeared to directly facilitate further capillary sprout growth. These cells incorporated into vascular endothelium near the sprout tip, co-expressing endothelial marker CD31. Sprouts had perfusion characteristics distinct from feeder vessels and many sprout tips were open-ended. CONCLUSIONS. Time-lapse in vivo corneal confocal microscopy can be used to track a temporal sequence of events in corneal angiogenesis. The technique has revealed potential roles for myeloid cells in promoting vessel sprouting in an inflammatory corneal setting.

Ämnesord och genrebeteckningar

MEDICINE
MEDICIN

Biuppslag (personer, institutioner, konferenser, titlar ...)

Fagerholm, PerÖstergötlands Läns Landsting,Linköpings universitet,Oftalmiatrik,Hälsouniversitetet,Ögonkliniken US/LiM(Swepub:liu)perfa04 (författare)
Traneus-Rockert, CatharinaÖstergötlands Läns Landsting,Klinisk patologi och klinisk genetik (författare)
Lagali, NeilÖstergötlands Läns Landsting,Linköpings universitet,Oftalmiatrik,Hälsouniversitetet,Ögonkliniken US/LiM(Swepub:liu)neina50 (författare)
Linköpings universitetOftalmiatrik (creator_code:org_t)

Sammanhörande titlar

Ingår i:Investigative Ophthalmology and Visual Science: Research in Vision and Opthalmology52:6, s. 3060-30680146-04041552-5783

Internetlänk

Hitta via bibliotek

Investigative Ophthalmology and Visual Science (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

1 av 1
Föregående post
Nästa post
Till träfflistan

Hitta mer i SwePub

Av författaren/redakt...: Bourghardt Peebo ...; Fagerholm, Per; Traneus-Rockert, ...; Lagali, Neil

Artiklar i publikationen: Investigative Op ...

Av lärosätet: Linköpings universitet

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se