Mouse corneal lymphangiogenesis model.

This protocol describes a powerful in vivo method to quantitatively study the formation of new lymphatic vessels in the avascular cornea without interference of pre-existing lymphatics. Implantation of 100 ng of lymphangiogenic factors such as vascular endothelial growth factor (VEGF)-A, VEGF-C or fibroblast growth factor-2, together with slow-release polymers, into a surgically created micropocket in the mouse cornea elicits a robust lymphangiogenic response. Newly formed lymphatic vessels are detected by immunohistochemical staining of the flattened corneal tissue with lymphatic endothelial-specific markers such as lymphatic vessel endothelial hyaluronan receptor-1; less-specific markers such as vascular endothelial growth factor receptor 3 may also be used. Lymphatic vessel growth in relation to hemangiogenesis can be readily detected starting at day 5 or 6 after pellet implantation and persists for ∼14 d. This protocol offers a unique opportunity to study the mechanisms underlying lymphatic vessel formation, remodeling and function.

Lim, SharonKarolinska Institutet (författare)
Ji, HongKarolinska Institutet (författare)
Zhang, YinKarolinska Institutet (författare)
Yang, YunlongKarolinska Institutet (författare)
Honek, JenniferKarolinska Institutet (författare)
Hedlund, Eva-MariaKarolinska Institutet (författare)
Cao, YihaiLinköpings universitet,Fysiologi,Hälsouniversitetet(Swepub:liu)yihca64 (författare)
Karolinska InstitutetFysiologi (creator_code:org_t)

Ingår i:Nature protocols: Springer Science and Business Media LLC6:6, s. 817-261750-27991754-2189

Av författaren/redakt...: Cao, Renhai; Lim, Sharon; Ji, Hong; Zhang, Yin; Yang, Yunlong; Honek, Jennifer; visa fler...; Hedlund, Eva-Mar ...; Cao, Yihai; visa färre...

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