Synthetic neoglycopolymer-recombinant human collagen hybrids as biomimetic crosslinking agents in corneal tissue engineering

Merrett, KimberleyDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada (författare)

Saturated neoglycopolymers, prepared via tandem ROMP-hydrogenation (ROMP = ring-opening metathesis polymerization) of carbohydrate-functionalized norbornenes, are investigated as novel collagen crosslinking agents in corneal tissue engineering. The neoglycopolymers were incorporated into recombinant human collagen type III (RHC III) as collagen crosslinking agents and glycosaminoglycan (GAG) mimics. The purely synthetic nature of these composites is designed to reduce susceptibility to immunological and allergic reactions, and to circumvent the transmission of animal infectious diseases. The collagen-neoglycopolymer biomaterials exhibit higher stability to collagenase-induced biodegradation than the control materials, composites of RHC III crosslinked using EDC/NHS (EDC = 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide; NHS = N-hydroxysuccinimide). Even at this proof of concept stage, the thermal stability, enzymatic resistance, and permeability of the neoglycopolymer hydrogels are comparable or superior to those of these fully optimized control materials, which have successfully been tested clinically. Tensile strength is adequate for transplantation, but lower than that of the optimized control materials.

Biomimetic materials; Crosslinking; Collagen; Cornea; Tissue engineering; Neoglycopolymer
TECHNOLOGY
TEKNIKVETENSKAP

Liu, WenguangDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada (författare)
Mitra, DebbieDepartment of Chemistry, Centre for Catalysis Research and Innovation, University of Ottawa, Ottawa, ON, Canada (författare)
Camm, Kenneth D.Department of Chemistry, Centre for Catalysis Research and Innovation, University of Ottawa, Ottawa, ON, Canada (författare)
McLaughlin, Christopher R.Department of Cellular and Molecular Medicine, University of Ottawa, and University of Ottawa Eye Institute, Ottawa, ON, Canada (författare)
Liu, YuwenUniversity of Ottawa Eye Institute, Ottawa, ON, Canada (författare)
Watsky, Mitchell A.Department of Physiology, University of Tennessee Health Sciences Centre, Memphis, TN, USA (författare)
Li, FengfuUniversity of Ottawa Eye Institute, Ottawa, ON, Canada (författare)
Griffith, MayDepartment of Cellular and Molecular Medicine, University of Ottawa, and University of Ottawa Eye Institute, Ottawa, ON, Canada(Swepub:liu)maygr76 (författare)
Fogg, Deryn E.Department of Chemistry, Centre for Catalysis Research and Innovation, University of Ottawa, Ottawa, ON, Canada (författare)
Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, CanadaDepartment of Chemistry, Centre for Catalysis Research and Innovation, University of Ottawa, Ottawa, ON, Canada (creator_code:org_t)

Av författaren/redakt...: Merrett, Kimberl ...; Liu, Wenguang; Mitra, Debbie; Camm, Kenneth D.; McLaughlin, Chri ...; Liu, Yuwen; visa fler...; Watsky, Mitchell ...; Li, Fengfu; Griffith, May; Fogg, Deryn E.; visa färre...

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