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Autosomal SNP typin...
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Tomas, CUniversity of Copenhagen
(författare)
Autosomal SNP typing of forensic samples with the GenPlex (TM) HID System: Results of a collaborative study
- Artikel/kapitelEngelska2011
Förlag, utgivningsår, omfång ...
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Elsevier,2011
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-70729
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-70729URI
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https://doi.org/10.1016/j.fsigen.2010.06.007DOI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Funding Agencies|Ellen and Aage Andersens Foundation||
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The GenPlex (TM) HID System (Applied Biosystems - AB) offers typing of 48 of the 52 SNPforID SNPs and amelogenin. Previous studies have shown a high reproducibility of the GenPlex (TM) HID System using 250500 pg DNA of good quality. An international exercise was performed by 14 laboratories (9 in Europe and 5 in the US) in order to test the robustness and reliability of the GenPlex (TM) HID System on forensic samples. Three samples with partly degraded DNA and 10 samples with low amounts of DNA were analyzed in duplicates using various amounts of DNA. In order to compare the performance of the GenPlex (TM) HID System with the most commonly used STR kits, 500 pg of partly degraded DNA from three samples was typed by the laboratories using one or more STR kits. The median SNP typing success rate was 92.3% with 500 pg of partly degraded DNA. Three of the fourteen laboratories counted for more than two thirds of the locus dropouts. The median percentage of discrepant results was 0.2% with 500 pg degraded DNA. An increasing percentage of locus dropouts and discrepant results were observed when lower amounts of DNA were used. Different success rates were observed for the various SNPs. The rs763869 SNP was the least successful. With the exception of the MiniFiler (TM) kit (AB), GenPlex (TM) HID performed better than five other tested STR kits. When partly degraded DNA was analyzed, GenPlex (TM) HID showed a very low mean mach probability, while all STR kits except MiniFiler (TM) had very limited discriminatory power.
Ämnesord och genrebeteckningar
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GenPlex (TM) HID
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Autosomal SNPs
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Forensic science
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Degraded DNA
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MEDICINE
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MEDICIN
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Axler-DiPerte, GNew York City Off Chief Med Examiner
(författare)
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Budimlija, Z MNew York City Off Chief Med Examiner
(författare)
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Borsting, CUniversity Copenhagen
(författare)
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Coble, M DArmed Forces Institute of Pathology
(författare)
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Decker, A EUS Natl Institute Stand and Technology
(författare)
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Eisenberg, AUniversity of North Texas Human Identification
(författare)
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Fang, RAppl Biosyst Inc
(författare)
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Fondevila, MUniversity Santiago de Compostela
(författare)
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Frisk Fredslund, SUniversity Copenhagen
(författare)
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Gonzalez, SUniversity of North Texas Human Identification
(författare)
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Hansen, A JUniversity Copenhagen
(författare)
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Hoff-Olsen, PUniversity of Oslo
(författare)
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Haas, CUniversity of Zurich
(författare)
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Kohler, PUniversity of Oslo
(författare)
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Kriegel, A KUniversity Hospital, Cologne
(författare)
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Lindblom, BertilLinköpings universitet,Rättsgenetik,Hälsouniversitetet(Swepub:liu)berli83
(författare)
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Manohar, FAppl Biosyst Inc
(författare)
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Maronas, OUniversity Santiago de Compostela
(författare)
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Mogensen, H SUniversity Copenhagen
(författare)
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Neureuther, KFed Criminal Police Off, Wiesbaden
(författare)
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Nilsson, HNational Board for Forensic Medicine
(författare)
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Scheible, M KArmed Forces Institute of Pathology
(författare)
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Schneider, P MUniversity Hospital, Cologne
(författare)
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Sonntag, M LFed Criminal Police Off, Wiesbaden
(författare)
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Stangegaard, MUniversity Copenhagen
(författare)
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Syndercombe-Court, DBarts and London Queen Marys School of Medicine and Dentistry
(författare)
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Thacker, C RBarts and London Queen Marys School of Medicine and Dentistry
(författare)
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Vallone, P MUS Natl Institute Stand and Technology
(författare)
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Westen, A ANetherlands Forensic Institute
(författare)
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Morling, NUniversity Copenhagen
(författare)
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University of CopenhagenNew York City Off Chief Med Examiner
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Forensic Science International: Elsevier5:5, s. 369-3751872-49731878-0326
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Till lärosätets databas
- Av författaren/redakt...
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Tomas, C
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Axler-DiPerte, G
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Budimlija, Z M
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Borsting, C
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Coble, M D
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Decker, A E
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visa fler...
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Eisenberg, A
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Fang, R
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Fondevila, M
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Frisk Fredslund, ...
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Gonzalez, S
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Hansen, A J
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Hoff-Olsen, P
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Haas, C
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Kohler, P
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Kriegel, A K
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Lindblom, Bertil
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Manohar, F
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Maronas, O
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Mogensen, H S
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Neureuther, K
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Nilsson, H
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Scheible, M K
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Schneider, P M
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Sonntag, M L
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Stangegaard, M
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Syndercombe-Cour ...
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Thacker, C R
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Vallone, P M
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Westen, A A
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Morling, N
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visa färre...
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Forensic Science ...
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Linköpings universitet