id:"swepub:oai:DiVA.org:liu-74635"
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- Lindqvist, MariaÖstergötlands Läns Landsting,Linköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet,Vårdhygien (author)
Detection and characterisation of SCCmec remnants in multiresistant methicillin-susceptible Staphylococcus aureus causing a clonal outbreak in a Swedish county
- Article/chapterEnglish2012
Publisher, publication year, extent ...
- 2011-05-18
- Springer Verlag (Germany),2012
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:liu-74635
- https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-74635URI
- https://doi.org/10.1007/s10096-011-1286-yDOI
Supplementary language notes
- Language:English
- Summary in:English
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- Funding Agencies|Ostergotland County Council||Scandinavian Society for Antimicrobial Chemotherapy (SSAC)|2009-22495|
- The purpose of this study was to investigate if multiresistant methicillin-susceptible Staphylococcus aureus (MR-MSSA) causing a clonal outbreak in A-stergotland County, Sweden, were derived from methicillin-resistant S. aureus (MRSA) by carrying remnants of SCCmec, and, if so, to characterise this element. A total of 54 MSSA isolates with concomitant resistance to erythromycin, clindamycin and tobramycin from 49 patients (91% clonally related, spa type t002) were investigated with the BD GeneOhm MRSA assay and real-time polymerase chain reaction (PCR) targeting the SCCmec integration site/SCCmec right extremity junction. DNA sequencing of one isolate representing the MR-MSSA outbreak clone was performed by massive parallel 454 pyrosequencing. All isolates that were part of the clonal outbreak carried SCCmec remnants. The DNA sequencing revealed the carriage of a pseudo-SCC element 12 kb in size, with a genomic organisation identical to an SCCmec type I (TM) I (TM) element, except for a 41-kb gap. This study demonstrates the presence of a pseudo-SCC element resembling SCCmec type II among MR-MSSA, suggesting possible derivation from MRSA. The presence of SCCmec remnants should always be considered when SCCmec typing is used for MRSA detection, and may not be suitable in locations with a high prevalence of MR-MSSA, since this might give a high number of false-positive results.
Subject headings and genre
- MEDICINE
- MEDICIN
Added entries (persons, corporate bodies, meetings, titles ...)
- Isaksson, BarbroÖstergötlands Läns Landsting,Linköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet,Vårdhygien(Swepub:liu)baris12 (author)
- Grub, C.Oslo University Hospital (author)
- Jonassen, T. Ö.Oslo University Hospital (author)
- Hällgren, AnitaÖstergötlands Läns Landsting,Linköpings universitet,Infektionsmedicin,Hälsouniversitetet,Infektionskliniken i Östergötland(Swepub:liu)aniha32 (author)
- Linköpings universitetKlinisk mikrobiologi (creator_code:org_t)
Related titles
- In:European Journal of Clinical Microbiology and Infectious Diseases: Springer Verlag (Germany)31:2, s. 141-1470934-97231435-4373
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