Sökning: WFRF:(Balao da Silva C M.) >
Toxicity of glycero...
Toxicity of glycerol for the stallion spermatozoa: Effects on membrane integrity and cytoskeleton, lipid peroxidation and mitochondrial membrane potential
-
- Macias Garcia, B (författare)
- University of Extremadura
-
- Ortega Ferrusola, C (författare)
- University of Extremadura
-
- Aparicio, I M (författare)
- University of Extremadura
-
visa fler...
-
- Miro-Moran, A (författare)
- University of Extremadura
-
- Morillo Rodriguez, A (författare)
- University of Extremadura
-
- Gallardo Bolanos, J M (författare)
- University of Extremadura
-
- Gonzalez Fernandez, L (författare)
- University of Extremadura
-
- Balao da Silva, C M (författare)
- University of Extremadura
-
- Rodriguez-Martinez, Heriberto (författare)
- Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet
-
- Tapia, J A (författare)
- University of Extremadura
-
- Pena, F J (författare)
- University of Extremadura
-
visa färre...
-
(creator_code:org_t)
- Elsevier, 2012
- 2012
- Engelska.
-
Ingår i: Theriogenology. - : Elsevier. - 0093-691X .- 1879-3231. ; 77:7, s. 1280-1289
- Relaterad länk:
-
https://liu.diva-por... (primary) (Raw object)
-
visa fler...
-
http://liu.diva-port...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Glycerol is, to date, the most widely used cryoprotectant to freeze stallion spermatozoa at concentrations between 2% and 5%. Cryoprotectant toxicity has been claimed to be the single most limiting factor for the success of cryopreservation. In order to evaluate the toxic effects of the concentrations of glycerol used in practice, stallion spermatozoa were incubated in Biggers Whitten and Whittingham (BWW) media supplemented with 0%, 0.5%, 1.5%, 2.5%, 3.5%, and 5% glycerol. In two additional experiments, a hyposmotic (75 mOsm/kg) and a hyperosmotic (900 mOsm/kg) control media were included. Sperm parameters evaluated included cell volume, membrane integrity, lipid peroxidation, caspase 3, 7, and 8 activation, mitochondrial membrane potential, and integrity of the cytoskeleton. Glycerol exerted toxicity at concentrations 3.5% and the maximal toxicity was observed at 5%. The actin cytoskeleton was especially sensitive to glycerol presence, inducing rapid F actin depolymerization at concentrations over 1.5%. The sperm membrane and the mitochondria were other structures affected. The toxicity of glycerol is apparently related to osmotic and nonosmotic effects. In view of our results the concentration of glycerol in the freezing media for stallion spermatozoa should not surpass 2.5%.
Nyckelord
- Stallion
- Sperm
- Glycerol
- Actin cytoskeleton
- Toxicity
- Cryopreservation
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas