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Susceptibility of C...
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Idh, JonnaLinköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
(författare)
Susceptibility of Clinical Strains of Mycobacterium tuberculosis to Reactive Nitrogen Species in Activated Macrophages
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-77131
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-77131URI
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:vet swepub-contenttype
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Ämneskategori:ovr swepub-publicationtype
Anmärkningar
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Background: Nitric oxide (NO) is produced in macrophages by the inducible NO synthase (iNOS) upon activation by pro-inflammatory cytokines. NO has been shown to be essential for the control of Mycobacterium tuberculosis infection in murine models whereas its importance in man is not as clear. There is a lack of studies regarding the susceptibility to reactive nitrogen species (RNS) in clinical strains of M. tuberculosis and the relation to first-line drug resistance, such as to isoniazid (INH). The aim of this study was to explore susceptibility to RNS and intracellular survival of clinical strains of M. tuberculosis, with or without INH resistance. Method: Seven clinical strains of M. tuberculosis were transformed with the pSMT1-plasmid encoding Vibrio harveyi luciferase. Survival was analysed by luminometry following exposure to the NO donor DETA/NO or peroxynitrite (SIN-1). Intracellular killing was studied in murine macrophages (RAW 264.7) activated with interferon gamma (IFN-γ) and lipopolysaccharide (LPS). Results: There was a significant effect on growth control of M. tuberculosis strains upon macrophage activation, which showed variability among clinical isolates. In the cell-free system, all strains showed a dose-dependent susceptibility to DETA/NO and SIN-1, and clinical strains were in general more resistant than H37Rv to DETA/NO. INH-resistant strains with an inhA mutation were significantly more tolerant to DETA/NO than inhA wild type. Conclusion: Reactive nitrogen species inhibited growth of clinical M. tuberculosis isolates both in an intra- and extracellular model with significant difference between strains. Increased tolerance to NO was associated with isoniazid resistance mediated by inhA.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Lerm, MariaLinköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet(Swepub:liu)marle69
(författare)
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Raffetseder, JohannaLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet(Swepub:liu)johra57
(författare)
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Eklund, DanielLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet(Swepub:liu)danek14
(författare)
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Larsson, MarieLinköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet(Swepub:liu)marla67
(författare)
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Pienaar, ElsjeLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet(Swepub:liu)elspi91
(författare)
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Forslund, TonyLinköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet(Swepub:liu)tonfo57
(författare)
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Werngren, JimDepartment of Preparedness, Unit of Highly Pathogenic Microorganisms, Swedish Institute for Communicable Disease Control, Sweden
(författare)
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Juréen, PontusDepartment of Preparedness, Swedish Institute for Communicable Disease Control, Sweden
(författare)
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Ängeby, KristianDepartment of Clinical Microbiology, Karolinska Institute, Karolinska University Hospital, Sweden
(författare)
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Sundqvist, TommyLinköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet(Swepub:liu)tomsu30
(författare)
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Stendahl, OlleLinköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet(Swepub:liu)ollst48
(författare)
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Schön, ThomasDepartment of Infectious Diseases and Clinical Microbiology, Kalmar County Hospital, Sweden
(författare)
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Linköpings universitetMedicinsk mikrobiologi
(creator_code:org_t)
Internetlänk
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Idh, Jonna
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Lerm, Maria
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Raffetseder, Joh ...
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Eklund, Daniel
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Larsson, Marie
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Pienaar, Elsje
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visa fler...
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Forslund, Tony
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Werngren, Jim
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Juréen, Pontus
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Ängeby, Kristian
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Sundqvist, Tommy
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Stendahl, Olle
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Schön, Thomas
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Linköpings universitet