In vitro Lymphocyte Proliferation as Compared to Patch Test Using Gold, Palladium and Nickel

Cederbrant, KarinLinköpings universitet,Kirurgi,Patologi,Hälsouniversitetet (author)

Background: A conventional lymphocyte transformation test (LTT) was compared to the commercially available MELISA® (memory lymphocyte immuno-stimulation assay), a lymphoproliferative assay that has been suggested to be a valuable instrument for the diagnosis of metal allergy. Sensitivity and specificity of the two assays were calculated using a patch test as a reference method.Methods: 34 patients were patch-tested for gold sodium thiosulfate, palladium chloride and nickel sulfate, and the lymphocyte proliferation to these metals was tested in vitro using mononuclear cells from peripheral blood.Results: No significant differences regarding sensitivity and specificity were found between MELISA and conventional LTT. The sensitivity varied between 55 and 95% and the specificity between 17 and 79%.Conclusions: The low specificity of the two in vitro assays suggests that they are not useful for diagnosis of contact allergy to the metals gold, palladium and nickel, since a large number of false-positive results will be obtained.

Hultman, PerLinköpings universitet,Patologi,Hälsouniversitetet(Swepub:liu)perhu66 (author)
Marcusson, Jan A.Department of Dermatology, Huddinge Hospital, Huddinge (author)
Tibbling, LitaLinköpings universitet,Oto-Rhino-Laryngologi,Hälsouniversitetet (author)
Linköpings universitetKirurgi (creator_code:org_t)

In:International Archives of Allergy and Immunology: S. Karger AG112:3, s. 212-2171018-24381423-0097

By the author/editor: Cederbrant, Kari ...; Hultman, Per; Marcusson, Jan A ...; Tibbling, Lita

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