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A Novel Class of An...
A Novel Class of Anti-HIV Agents with Multiple Copies of Enfuvirtide Enhances Inhibition of Viral Replication and Cellular Transmission In Vitro
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- Chang, Chien-Hsing (författare)
- Morris Plains, New Jersey, United States of America
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- Hinkula, Jorma (författare)
- Linköpings universitet,Molekylär virologi,Hälsouniversitetet
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- Loo, Meiyu (författare)
- Morris Plains, New Jersey, United States of America
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- Falkeborn, Tina (författare)
- Linköpings universitet,Molekylär virologi,Hälsouniversitetet
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- Li, Rongxiu (författare)
- Morris Plains, New Jersey, United States of America
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- Cardillo, Thomas M. (författare)
- Morris Plains, New Jersey, United States of America
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- Rossi, Edmund A. (författare)
- Morris Plains, New Jersey, United States of America
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- Goldenberg, David M. (författare)
- Morris Plains, New Jersey, United States of America
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- Wahren, Britta (författare)
- Karolinska Institutet,Karolinska Institutet, Stockholm, Sweden
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(creator_code:org_t)
- 2012-07-23
- 2012
- Engelska.
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 7:7, s. e41235-e41235
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https://liu.diva-por... (primary) (Raw object)
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https://journals.plo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
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- We constructed novel HIV-1 fusion inhibitors that may overcome the current limitations of enfuvirtide, the first such therapeutic in this class. The three prototypes generated by the Dock-and-Lock (DNL) technology to comprise four copies of enfuvirtide tethered site-specifically to the Fc end of different humanized monoclonal antibodies potently neutralize primary isolates (both R5-tropic and X4-tropic), as well as T-cell-adapted strains of HIV-1 in vitro. All three prototypes show EC50 values in the subnanomolar range, which are 10- to 100-fold lower than enfuvirtide and attainable whether or not the constitutive antibody targets HIV-1. The potential of such conjugates to purge latently infected cells was also demonstrated in a cell-to-cell viral inhibition assay by measuring their efficacy to inhibit the spread of HIV-1LAI from infected human peripheral blood mononuclear cells to Jurkat T cells over a period of 30 days following viral activation with 100 nM SAHA (suberoylanilide hydroxamic acid). The IgG-like half-life was not significantly different from that of the parental antibody, as shown by the mean serum concentration of one prototype in mice at 72 h. These encouraging results provide a rationale to develop further novel anti-HIV agents by coupling additional antibodies of interest with alternative HIV-inhibitors via recombinantly-produced, self-assembling, modules.
Nyckelord
- MEDICINE
- MEDICIN
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- art (ämneskategori)
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