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Growth Factor Relea...
Growth Factor Release during Preparation and Storage of Platelet Concentrates
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- Ledent, Elisabeth (författare)
- Linköpings universitet,Östergötlands Läns Landsting,Transfusionsmedicin och klinisk immunologi,Cellbiologi,Hälsouniversitetet
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- Wasteson, Åke (författare)
- Linköpings universitet,Cellbiologi,Hälsouniversitetet
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- Berlin, Gösta (författare)
- Linköpings universitet,Östergötlands Läns Landsting,Transfusionsmedicin och klinisk immunologi,Hälsouniversitetet
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(creator_code:org_t)
- Wiley, 1995
- 1995
- Engelska.
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Ingår i: Vox Sanguinis. - : Wiley. - 0042-9007 .- 1423-0410. ; 68:4, s. 205-209
- Relaterad länk:
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https://urn.kb.se/re...
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visa fler...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The platelet content of platelet-derived growth factor (PDGF), a mitogen stored in the alpha-granules, was studied during preparation and storage of platelet concentrates (PC) and compared to the growth-promoting activity of platelets, β-thromboglobulin (β-TG) and lactate dehydrogenase (LD). We compared PC prepared from platelet-rich plasma (PRP-PC; n= 10) and from buffy coat. Two different pre-preparation storage periods of the buffy coat were used: 4h (BC-PC:4h; n = 10) and 24 h (BC-PC: 24h; n = 5). The platelet content of PDGF and β-TG was measured by a RIA technique and the growth-promoting activity by incorporation of 3H-thymidine in stimulated fibroblasts. The platelet content of PDGF, β-TG and the growth-promoting activity of the platelets decreased in a similar way during preparation and storage of PRP-PC (31 ±2, 35±2 and 33±7%, respectively, at day 5 of storage; mean ± SEM). The release of LD was minor (3.9 ±0.5% at day 5). At day 1 of storage the platelet content of PDGF was significantly better preserved in BC-PC:4h than in BD-PC:24h (88±2 and 81 ±3%, respectively; p = 0.03). Comparing BC-PC:4h and PRP-PC we found a significantly better preservation of PDGF in BC-PC:4h until day 3 of storage (80±2 and 75±1%, respectively at day 3; p = 0.046). In conclusion the preparation of PC according to the PRP method initially induces a higher loss of PDGF, and hence of the growth-promoting activity, than the BC method.
Nyckelord
- MEDICINE
- MEDICIN
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- art (ämneskategori)
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