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Neutralization of m...
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Diab, AsimDivision of Neurology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden
(författare)
Neutralization of macrophage inflammatory protein 2 (MIP-2) and MIP-1α attenuates neutrophil recruitment in the central nervous system during experimental bacterial meningitis
- Artikel/kapitelEngelska1999
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-81946
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-81946URI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1946632URI
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https://doi.org/10.1128/IAI.67.5.2590-2601.1999DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Chemokines are low-molecular-weight chemotactic cytokines that have been shown to play a central role in the perivascular transmigration and accumulation of specific subsets of leukocytes at sites of tissue damage. Using in situ hybridization (ISH), we investigated the mRNA induction of macrophage inflammatory protein 2 (MIP-2), MIP-1α, monocyte chemoattractant protein 1 (MCP-1), and RANTES. Challenge of infant rats’ brains with Haemophilus influenzae type b intraperitoneally resulted in the time-dependent expression of MIP-2, MIP-1α, MCP-1, and RANTES, which was maximal 24 to 48 h postinoculation. Immunohistochemistry showed significant increases in neutrophils and macrophages infiltrating the meninges, the ventricular system, and the periventricular area. The kinetics of MIP-2, MIP-1α, MCP-1, and RANTES mRNA expression paralleled those of the recruitment of inflammatory cells and disease severity. Administration of anti-MIP-2 or anti-MIP-1α antibodies (Abs) resulted in significant reduction of neutrophils. Administration of anti-MCP-1 Abs significantly decreased macrophage infiltration. Combined studies of ISH and immunohistochemistry showed that MIP-2- and MIP-1α-positive cells were neutrophils and macrophages. MCP-1-positive cells were neutrophils, macrophages, and astrocytes. Expression of RANTES was localized predominantly to resident astrocytes and microglia. The present study indicates that blocking of MIP-2 or MIP-1α bioactivity in vivo results in decreased neutrophil influx. These data are also the first demonstration that the C-C chemokine MIP-1α is involved in neutrophil recruitment in vivo.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Abdalla, HanaDivision of Infectious Diseases, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden(Swepub:liu)hanab12
(författare)
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Lun Li, HuDivision of Neurology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden
(författare)
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Dong Shi, FuDivision of Neurology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden
(författare)
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Zhu, JieKarolinska Institutet
(författare)
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Höjberg, BoDivision of Neurology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden
(författare)
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Lindquist, LarsKarolinska Institutet
(författare)
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Wretlind, BengtDivision of Clinical Bacteriology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden
(författare)
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Bakhiet, MoizDivision of Infectious Diseases, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden
(författare)
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Link, HansKarolinska Institutet
(författare)
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Division of Neurology, Karolinska Institute, Huddinge University Hospital, Stockholm, SwedenDivision of Infectious Diseases, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Infection and Immunity67:5, s. 2590-26010019-95671098-5522
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Till lärosätets databas
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Diab, Asim
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Abdalla, Hana
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Lun Li, Hu
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Dong Shi, Fu
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Zhu, Jie
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Höjberg, Bo
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visa fler...
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Lindquist, Lars
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Wretlind, Bengt
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Bakhiet, Moiz
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Link, Hans
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visa färre...
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Infection and Im ...
- Av lärosätet
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Linköpings universitet
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Karolinska Institutet