Search: onr:"swepub:oai:DiVA.org:liu-84885" >
Lipopolysaccharide-...
-
Engström, LindaLinköpings universitet,Cellbiologi,Hälsouniversitetet
(author)
Lipopolysaccharide-Induced Fever Depends on Prostaglandin E2 Production Specifically in Brain Endothelial Cells
- Article/chapterEnglish2012
Publisher, publication year, extent ...
-
Endocrine Society,2012
-
electronicrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:liu-84885
-
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-84885URI
-
https://doi.org/10.1210/en.2012-1375DOI
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:125307467URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Funding Agencies|Swedish Research Council|7879|Swedish Cancer Foundation|100533|Swedish Brain Foundation||Gustav V:s 80-ars Fond||
-
Immune-induced prostaglandin E2 (PGE2) synthesis is critical for fever and other centrally elicited disease symptoms. The production of PGE2 depends on cyclooxygenase-2 and microsomal prostaglandin E synthase-1 (mPGES-1), but the identity of the cells involved has been a matter of controversy. We generated mice expressing mPGES-1 either in cells of hematopoietic or nonhematopoietic origin. Mice lacking mPGES-1 in hematopoietic cells displayed an intact febrile response to lipopolysaccharide, associated with elevated levels of PGE2 in the cerebrospinal fluid. In contrast, mice that expressed mPGES-1 only in hematopoietic cells, although displaying elevated PGE2 levels in plasma but not in the cerebrospinal fluid, showed no febrile response to lipopolysaccharide, thus pointing to the critical role of brain-derived PGE2 for fever. Immunohistochemical stainings showed that induced cyclooxygenase-2 expression in the brain exclusively occurred in endothelial cells, and quantitative PCR analysis on brain cells isolated by flow cytometry demonstrated that mPGES-1 is induced in endothelial cells and not in vascular wall macrophages. Similar analysis on liver cells showed induced expression in macrophages and not in endothelial cells, pointing at the distinct role for brain endothelial cells in PGE2 synthesis. These results identify the brain endothelial cells as the PGE2-producing cells critical for immune-induced fever.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Ruud, JohanLinköpings universitet,Cellbiologi,Hälsouniversitetet(Swepub:liu)johru61
(author)
-
Eskilsson, AnnaLinköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet(Swepub:liu)annes38
(author)
-
Larsson, AndersLinköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet(Swepub:liu)andla36
(author)
-
Mackerlova, LudmilaLinköpings universitet,Cellbiologi,Hälsouniversitetet(Swepub:liu)ludma70
(author)
-
Kugelberg, UnnLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet
(author)
-
Qian, HongKarolinska Institutet,Linköpings universitet,Experimentell hematologi,Hälsouniversitetet(Swepub:liu)honqi03
(author)
-
Vasilache, Ana MariaLinköpings universitet,Cellbiologi,Hälsouniversitetet(Swepub:liu)anava88
(author)
-
Larsson, PeterLinköpings universitet,Medicinsk radiofysik,Hälsouniversitetet(Swepub:liu)petla27
(author)
-
Engblom, DavidLinköpings universitet,Cellbiologi,Hälsouniversitetet(Swepub:liu)daven69
(author)
-
Sigvardsson, MikaelLinköpings universitet,Experimentell hematologi,Hälsouniversitetet(Swepub:liu)miksi32
(author)
-
Jönsson, Jan-IngvarLinköpings universitet,Experimentell hematologi,Hälsouniversitetet(Swepub:liu)janjo86
(author)
-
Blomqvist, AndersLinköpings universitet,Cellbiologi,Hälsouniversitetet(Swepub:liu)andbl47
(author)
-
Linköpings universitetCellbiologi
(creator_code:org_t)
Related titles
-
In:Endocrinology: Endocrine Society153:10, s. 4849-48610013-72271945-7170
Internet link
Find in a library
To the university's database
- By the author/editor
-
Engström, Linda
-
Ruud, Johan
-
Eskilsson, Anna
-
Larsson, Anders
-
Mackerlova, Ludm ...
-
Kugelberg, Unn
-
show more...
-
Qian, Hong
-
Vasilache, Ana M ...
-
Larsson, Peter
-
Engblom, David
-
Sigvardsson, Mik ...
-
Jönsson, Jan-Ing ...
-
Blomqvist, Ander ...
-
show less...
- Articles in the publication
-
Endocrinology
- By the university
-
Linköping University
-
Karolinska Institutet