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Alterations of the ...
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Kondratov, Aleksandr GNational Academic Science Ukraine, Ukraine
(författare)
Alterations of the WNT7A Gene in Clear Cell Renal Cell Carcinomas
- Artikel/kapitelEngelska2012
Förlag, utgivningsår, omfång ...
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2012-10-08
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Public Library of Science,2012
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-85296
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-85296URI
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https://doi.org/10.1371/journal.pone.0047012DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:125437894URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Funding Agencies|State Fond of Fundamental Research|F46/457-2011|Swedish Cancer Society||Swedish Institute||Swedish Research Council||EACR||
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WNT7A (wingless-type MMTV integration site family, member 7A) is a known tumor suppressor gene of non-small cell lung carcinomas (NSCLC) and is frequently inactivated due to CpG-island hypermethylation in human cancers. The members of WNT family are involved in cell signaling and play crucial roles in cancer development. In the present work hypermethylation of the WNT7A gene was detected in 66% (29/44) of analyzed clear cell renal cell carcinomas (RCCs) using methyl-specific PCR (MSP). Moreover, bisulfite sequencing confirmed intensive hypermethylation of the 5-CpG island of the WNT7A gene. Methylation analysis revealed positive correlations between tumor stage, Fuhrman nuclear grade and WNT7A hypermethylation. Additionally, restoration of WNT7A gene expression in the A498 cell line by 5-aza-2-deoxycytidine treatment confirmed a direct contribution of hypermethylation in silencing of the WNT7A gene. High frequency of loss of heterozygosity (LOH) was demonstrated on chromosome 3p25 in regions surrounding the WNT7A gene. The frequent down-regulation of WNT7A gene expression was detected in 88% (15/17) of clear cell RCCs. We have also shown that the WNT7A gene possesses tumor suppression function by colony-formation and cell proliferation assays in RCC cell lines. In summary, the WNT7A gene is inactivated by genetic/epigenetic alterations in clear cell RCC and demonstrates tumor suppressor properties.
Ämnesord och genrebeteckningar
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TECHNOLOGY
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TEKNIKVETENSKAP
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Kvasha, Sergiy MNational Academic Science Ukraine, Ukraine
(författare)
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Stoliar, Liubov ANational Academic Science Ukraine, Ukraine
(författare)
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Romanenko, Alina MUkraine Academic Medical Science, Ukraine
(författare)
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Zgonnyk, Yury MUkraine Academic Medical Science, Ukraine
(författare)
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Gordiyuk, Vasily VNational Academic Science Ukraine, Ukraine
(författare)
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Kashuba, Elena VKarolinska Institutet
(författare)
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Rynditch, Alla VNational Academic Science Ukraine, Ukraine
(författare)
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Zabarovsky, Eugene RLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet
(författare)
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Kashuba, Vladimir IKarolinska Institutet
(författare)
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National Academic Science Ukraine, UkraineUkraine Academic Medical Science, Ukraine
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:PLOS ONE: Public Library of Science7:101932-6203
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Kondratov, Aleks ...
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Kvasha, Sergiy M
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Stoliar, Liubov ...
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Romanenko, Alina ...
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Zgonnyk, Yury M
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Gordiyuk, Vasily ...
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Kashuba, Elena V
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Rynditch, Alla V
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Zabarovsky, Euge ...
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Kashuba, Vladimi ...
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