Tryptophan-accelerated electron flow through proteins

Shih, CrystalBeckman Institute, California Institute of Technology, Pasadena, USA (author)

Energy flow in biological structures often requires submillisecond charge transport over long molecular distances. Kinetics modeling suggests that charge-transfer rates can be greatly enhanced by multistep electron tunneling in which redox-active amino acid side chains act as intermediate donors or acceptors. We report transient optical and infrared spectroscopic experiments that quantify the extent to which an intervening tryptophan residue can facilitate electron transfer between distant metal redox centers in a mutant Pseudomonas aeruginosa azurin. CuI oxidation by a photoexcited ReI-diimine at position 124 on a histidine(124)-glycine(123)-tryptophan(122)-methionine(121) β strand occurs in a few nanoseconds, fully two orders of magnitude faster than documented for single-step electron tunneling at a 19 angstrom donor-acceptor distance.

Museth, Anna KatrineBeckman Institute, California Institute of Technology, Pasadena, USA(Swepub:liu)annmu48 (author)
Abrahamsson, MalinBeckman Institute, California Institute of Technology, Pasadena, USA (author)
Blanco-Rodriguez, Ana MariaSchool of Biological and Chemical Sciences, Queen Mary, University of London, London, UK (author)
Di Bilio, Angel J.Beckman Institute, California Institute of Technology, Pasadena, USA (author)
Sudhamsu, JawaharDepartment of Chemistry and Chemical Biology, Cornell University, Ithaca, USA (author)
Crane, Brian R.Department of Chemistry and Chemical Biology, Cornell University, Ithaca, USA (author)
Ronayne, Kate L.Central Laser Facility, STFC Rutherford Appleton Laboratory, Chilton, Didcot, Oxfordshire, UK (author)
Towrie, MikeCentral Laser Facility, STFC Rutherford Appleton Laboratory, Chilton, Didcot, Oxfordshire, UK (author)
Vlček Jr., AntoninSchool of Biological and Chemical Sciences, Queen Mary, University of London, London, UK (author)
Richards, John H.Beckman Institute, California Institute of Technology, Pasadena, USA (author)
Winkler, Jay R.Beckman Institute, California Institute of Technology, Pasadena, USA (author)
Gray, Harry B.Beckman Institute, California Institute of Technology, Pasadena, USA (author)
Beckman Institute, California Institute of Technology, Pasadena, USASchool of Biological and Chemical Sciences, Queen Mary, University of London, London, UK (creator_code:org_t)

In:Science: American Association for the Advancement of Science (AAAS)320:5884, s. 1760-17620036-80751095-9203

By the author/editor: Shih, Crystal; Museth, Anna Kat ...; Abrahamsson, Mal ...; Blanco-Rodriguez ...; Di Bilio, Angel ...; Sudhamsu, Jawaha ...; show more...; Crane, Brian R.; Ronayne, Kate L.; Towrie, Mike; Vlček Jr., Anton ...; Richards, John H ...; Winkler, Jay R.; Gray, Harry B.; show less...

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