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Evidence for radios...
Evidence for radiosensitizing by gliotoxin in HL-60 cells : implications for a role of NF-kappa B independent mechanisms
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- Baust, H. (författare)
- Department of Radiation Oncology, University of Ulm, D-89081 Ulm, Germany
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- Schoke, A. (författare)
- Department of Internal Medicine, University of Ulm, D-89081 Ulm, Germany
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- Brey, A. (författare)
- Department of Internal Medicine, University of Ulm, D-89081 Ulm, Germany
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- Gern, U. (författare)
- Department of Internal Medicine, University of Ulm, D-89081 Ulm, Germany
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- Los, Marek Jan (författare)
- Institute of Experimental Dermatology, University of Muenster, D-48149 Muenster, Germany
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- Schmid, R. M. (författare)
- 2nd Department of Internal Medicine, University of Munich, D-81675 Munich, Germany
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- Röttinger, E. M. (författare)
- Department of Radiation Oncology, University of Ulm, D-89081 Ulm, Germany
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- Seufferlein, T. (författare)
- Department of Internal Medicine, University of Ulm, D-89081 Ulm, Germany
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(creator_code:org_t)
- 2003-11-27
- 2003
- Engelska.
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Ingår i: Oncogene. - : Springer Science and Business Media LLC. - 0950-9232 .- 1476-5594. ; 22:54, s. 8786-8796
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Radioresistance markedly impairs the efficacy of tumor radiotherapy and may involve antiapoptotic signal transduction pathways that prevent radiation-induced cell death. A common cellular response to genotoxic stress induced by radiation is the activation of the nuclear factor kappa B (NF-kappaB). NF-kappaB activation in turn can lead to an inhibition of radiation-induced apoptotic cell death. Thus, inhibition of NF-kappaB activation is commonly regarded as an important strategy to abolish radioresistance. Among other compounds, the fungal metabolite gliotoxin (GT) has been reported to be a highly selective inhibitor of NF-kappaB activation. Indeed, low doses of GT were sufficient to significantly enhance radiation-induced apoptosis in HL-60 cells. However, this effect turned out to be largely independent of NF-kappaB activation since radiation of HL-60 cells with clinically relevant doses of radiation induced only a marginal increase in NF-kappaB activity, and selective inhibition of NF-kappaB by SN50 did not result in a marked enhancement of GT-induced apoptosis. GT induced activation of JNKs, cytochrome c release from the mitochondria and potently stimulated the caspase cascade inducing cleavage of caspases -9, -8, -7 and -3. Furthermore, cleavage of the antiapoptotic protein X-linked IAP and downregulation of the G2/M-specific IAP-family member survivin were observed during GT-induced apoptosis. Finally, the radiation-induced G2/M arrest was markedly reduced in GT-treated cells most likely due to the rapid induction of apoptosis. Our data demonstrate that various other pathways apart from the NF-kappaB signaling complex can sensitize tumor cells to radiation and propose a novel mechanism for radio-sensitization by GT, the interference with the G2/M checkpoint that is important for repair of radiation-induced DNA damage in p53-deficient tumor cells.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- 26s proteasome
- c-jun
- cytochrome-c
- death
- gliotoxin and radiosensitizing
- induction
- inhibition
- ionizing-radiation
- jnk
- NF-kappa B
- persistent activation
- protein-kinase pathway
- radiation-induced apoptosis
- survivin
- XIAP
Publikations- och innehållstyp
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- art (ämneskategori)
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