An Fcγ receptor I (CD64)-negative subpopulation of human peripheral blood monocytes is resistant to killing by antigen-activated CD4-positive cytotoxic T cells

Grage-Griebenow, E.Forschungszentrum Borstel, Department of Immunology and Cell Biology, Borstel, Germany (author)

It has been demonstrated that in monocyte/T cell co-cultures activated with recall antigens, cytotoxic T cells were generated which are able to reduce the number of antigen-presenting monocytes. In previous studies we could show that a minor subset of monocytes, the Fc gamma receptor I-negative (CD64(-)) monocytes, exhibits significantly higher antigen-presenting capacity than the main population of monocytes (> 90%) which are Fc gamma receptor I-positive (CD64(+)). Therefore, we addressed the question whether they are also differentially susceptible to T cell-mediated killing. In the present study we demonstrate that the CD64(-) monocyte subset is more resistant to killing by antigen-activated T cells than CD64(+) monocytes, as indicated by a higher viability and recovery of CD64(-) monocytes. This mechanism involves CD95 (Fas) antigen, since monocyte death in co-cultures with antigen-activated T cells could be partially reduced by blocking anti-Fas monoclonal antibodies (mAb). In agreement with this finding, although CD95 antigen was expressed on CD64(+) and CD64(-) monocytes at comparable levels, killing of CD64(-) monocytes by activating anti-Fas mAb was lower than of CD64(+) monocytes.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
NATURVETENSKAP Biologi Immunologi hsv//swe
NATURAL SCIENCES Biological Sciences Immunology hsv//eng
Apoptosis
CD64-negative monocytes
cytotoxic T cell
dendritic cells
differential expression
enzyme-like protease
fas-mediated apoptosis
flow-cytometry
ifn-alpha
lymphocytes
macrophages
monoclonal-antibodies
presenting cells
recall antigen
resistance to killing

Baran, J.Jagiellonian University, Institute of Molecular Biology, Cracow, Poland (author)
Loppnow, H.Forschungszentrum Borstel, Department of Immunology and Cell Biology, Borstel, Germany (author)
Los, Marek JanDeutsches Krebsforschungs-zentrum, Heidelberg, Germany(Swepub:liu)marlo14 (author)
Ernst, M.Forschungszentrum Borstel, Department of Immunology and Cell Biology, Borstel, Germany (author)
Flad, H. D.Forschungszentrum Borstel, Department of Immunology and Cell Biology, Borstel, Germany (author)
Pryjma, J.Jagiellonian University, Institute of Molecular Biology, Cracow, Poland (author)
Forschungszentrum Borstel, Department of Immunology and Cell Biology, Borstel, GermanyJagiellonian University, Institute of Molecular Biology, Cracow, Poland (creator_code:org_t)

By the author/editor: Grage-Griebenow, ...; Baran, J.; Loppnow, H.; Los, Marek Jan; Ernst, M.; Flad, H. D.; show more...; Pryjma, J.; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Immunology

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