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Functional gene tra...
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Spetz, Anna-LenaKarolinska Institutet
(författare)
Functional gene transfer of HIV DNA by an HIV receptor-independent mechanism
- Artikel/kapitelEngelska1999
Förlag, utgivningsår, omfång ...
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American Association of Immunologists,1999
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LIBRIS-ID:oai:DiVA.org:liu-88116
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-88116URI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:15843085URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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HIV-1 enters target cells mainly via binding to CD4 and its coreceptors. The presence of HIV-1 in CD4(-) cells suggests, however, that there exist other mechanisms for viral entry, Here it is reported that HIV-1 DNA may be transferred from one cell to another by uptake of apoptotic bodies in a CD4-independent way. This was investigated by coculturing CD4(-), chemokine receptor CCR5(-) and CXCR4(-) human fetal fibroblasts with apoptotic HIV-1-infected HuT78 cells or apoptotic PBMC isolated from HIV-1-infected patients. After 2 wk of coculture, fibroblasts contained HIV-1 DNA and expressed HIV-1 proteins p24 and gp120, Transfer of HIV-1 DNA was verified by coculturing fibroblasts with apoptotic bodies derived from cells infected with a defective HIV-1 virus. These cells contain one integrated copy of a reverse transcriptase (RT)-negative HIV-1 strain (8E5/LAV RT- cells) and consequently cannot produce free virus, Intracellular HIV-1 gag DNA was detected in both fibroblasts and dendritic cells after coculture with apoptotic 8E5/LAV RT- cells. Transfer of viral DNA after uptake of apoptotic bodies mag explain HIV-1 infection of CD4(-) cells in vivo and furthermore may be relevant for Ag presentation.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Patterson, Bruce K.Northwestern University Medical School, Chicago, USA
(författare)
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Lore, KarinKarolinska Institutet
(författare)
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Andersson, JanKarolinska Institutet
(författare)
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Holmgren, LarsKarolinska Institutet
(författare)
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Karolinska InstitutetNorthwestern University Medical School, Chicago, USA
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Immunology: American Association of Immunologists163:2, s. 736-7420022-17671550-6606
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