Sökning: L773:0003 2670 OR L773:1873 4324
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Determination of ur...
Determination of urea with an ammonia gas-sensitive semiconductor device in combination with urease
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- Winquist, Fredrik (författare)
- Linköpings universitet,Tillämpad Fysik,Tekniska högskolan
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- Spetz, Anita (författare)
- Linköpings universitet,Tillämpad Fysik,Tekniska högskolan
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- Lundström, Ingemar (författare)
- Linköpings universitet,Tillämpad Fysik,Tekniska högskolan
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- Danielsson, B. (författare)
- University of Lund, Sweden
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(creator_code:org_t)
- Elsevier, 1984
- 1984
- Engelska.
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Ingår i: Analytica Chimica Acta. - : Elsevier. - 0003-2670 .- 1873-4324. ; 163, s. 143-149
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- An ammonia gas-sensitive Ir/Pd MOS capacitor is used for urea determinations with the aid of urease in two different systems. One combination utilizes a reaction column with immobilized urease in a flow-injection system. The lower limit of urea detection for 150-μl samples was 0.2 μM. Urea in whole blood and blood serum was determined after a 500-fold dilution, and 15 samples per hour could be assayed. The relative standard deviation was 4.6% (n=10). Recovery tests were satisfactory. Values obtained for urea in serum correlated well with those from a spectrophotometric method. The other combination is based on a small flow cell with free urease enclosed between a dialysis membrane and a gas-permeable membrane. Urea was determined in the concentration range 0.01–50 mM. The enzyme probe could be used for up to four days without changes of behaviour.
Nyckelord
- TECHNOLOGY
- TEKNIKVETENSKAP
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