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- Sommar, PehrLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet (author)
Osteogenically-induced human dermal fibroblasts as a tool to regenerate bone
- Article/chapterEnglish2013
Publisher, publication year, extent ...
- Informa Healthcare,2013
- printrdacarrier
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- LIBRIS-ID:oai:DiVA.org:liu-89513
- https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-89513URI
- https://doi.org/10.3109/2000656X.2012.731411DOI
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- Language:English
- Summary in:English
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Notes
- Engineering of bone tissue could help to overcome the need for extensive reconstruction and associated donor site morbidity, and it has been proposed that osteogenic biomaterials, which are scaffolds that contain osteocompetent cells, could be used to fill large bone defects. This study investigated the potential of osteogenically-induced human dermal fibroblasts cultured on gelatin microcarriers combined with platelet-rich plasma in a model of a femoral defect in athymic rats. Defects were transplanted with one of the following six combinations: 1 = sodium chloride, 2 = platelet-rich plasma, 3 = microcarriers + platelet-rich plasma, 4 = human dermal fibroblasts on microcarriers + platelet-rich plasma, 5 = human osteoblasts on microcarriers + platelet-rich plasma, and 6 = osteogenically induced human dermal fibroblasts on microcarriers + platelet-rich plasma. The femoral defects were assessed 4 weeks postoperatively with computed tomography (CT), routine histological staining, fluorescence in situ hybridisation, and polyclonal antibodies directed towards osteocalcin and osteonectin. Radiographs of all groups taken 4 weeks postoperatively showed unhealed defects. Femoral defects transplanted with osteogenically-induced human dermal fibroblasts on microcarriers (group 6) contained dense clusters of cells with large quantities of extracellular matrix. These clusters were exclusive to this group and stained strongly for osteocalcin and osteonectin. Fluorescence in situ hybridisation showed viable human cells in femoral defects that had been transplanted with microcarriers seeded with cells, which confirmed the survival of implanted cells. In conclusion, osteogenically-induced human dermal fibroblasts survived in this new niche, and bone-like structures were apparent in the defects.
Subject headings and genre
- Tissue engineering
- stem cells
- human dermal fibroblasts
- differentiation
- osteogenic induction
- formation of bone
- MEDICINE
- MEDICIN
Added entries (persons, corporate bodies, meetings, titles ...)
- Junker, JohanLinköpings universitet,Cellbiologi,Hälsouniversitetet(Swepub:liu)johju92 (author)
- Strandenes, EivindHaukeland Hospital, Norway (author)
- Ness, CharlotteHaukeland Hospital, Norway (author)
- Hansson, ThomasÖstergötlands Läns Landsting,Linköpings universitet,Kirurgi,Hälsouniversitetet,Hand- och plastikkirurgiska kliniken US(Swepub:liu)thoha80 (author)
- Johnson, HansÖstergötlands Läns Landsting,Linköpings universitet,Kirurgi,Hälsouniversitetet,Hand- och plastikkirurgiska kliniken US(Swepub:liu)hanjo30 (author)
- Kratz, GunnarÖstergötlands Läns Landsting,Linköpings universitet,Hand och plastikkirurgi,Hälsouniversitetet,Hand- och plastikkirurgiska kliniken US(Swepub:liu)gunkr37 (author)
- Linköpings universitetInstitutionen för klinisk och experimentell medicin (creator_code:org_t)
Related titles
- In:Journal of Plastic Surgery and Hand Surgery: Informa Healthcare47:1, s. 8-132000-656X2000-6764
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