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Importance of havin...
Importance of having low-density functional groups for generating high-performance semiconducting polymer dots
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- Zhang, Xuanjun (författare)
- Department of Chemistry, University of Washington, Seattle, USA
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- Yu, Jiangbo (författare)
- Department of Chemistry, University of Washington, Seattle, USA
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- Wu, Chengfen g (författare)
- Department of Chemistry, University of Washington, Seattle, USA
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- Jin, Yuhui (författare)
- Department of Chemistry, University of Washington, Seattle, USA
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- Rong, Yu (författare)
- Department of Chemistry, University of Washington, Seattle, USA
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- Ye, Fangmao (författare)
- Department of Chemistry, University of Washington, Seattle, USA
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- Chiu, Daniel T (författare)
- Department of Chemistry, University of Washington, Seattle, USA
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(creator_code:org_t)
- 2012-05-24
- 2012
- Engelska.
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Ingår i: ACS Nano. - : American Chemical Society (ACS). - 1936-0851 .- 1936-086X. ; 6:6, s. 5429-5439
- Relaterad länk:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Semiconducting polymers with low-density side-chain carboxylic acid groups were synthesized to form stable, functionalized, and highly fluorescent polymer dots (Pdots). The influence of the molar fraction of hydrophilic side-chains on Pdot properties and performance was systematically investigated. Our results show that the density of side-chain carboxylic acid groups significantly affects Pdot stability, internal structure, fluorescence brightness, and nonspecific binding in cellular labeling. Fluorescence spectroscopy, single-particle imaging, and a dye-doping method were employed to investigate the fluorescence brightness and the internal structure of the Pdots. The results of these experiments indicate that semiconducting polymers with low density of side-chain functional groups can form stable, compact, and highly bright Pdots as compared to those with high density of hydrophilic side-chains. The functionalized polymer dots were conjugated to streptavidin (SA) by carbodiimide-catalyzed coupling and the Pdot-SA probes effectively and specifically labeled the cancer cell-surface marker Her2 in human breast cancer cells. The carboxylate-functionalized polymer could also be covalently modified with small functional molecules to generate Pdot probes for click chemistry-based bio-orthogonal labeling. This study presents a promising approach for further developing functional Pdot probes for biological applications.
Nyckelord
- Semiconducting polymer dots
- specific cellular targeting
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