id:"swepub:oai:DiVA.org:liu-90744"
Sökning: id:"swepub:oai:DiVA.org:liu-90744" > Isozyme profile and...
- 1 av 1
- Föregående post
- Nästa post
- Till träfflistan
- Linder, CeciliaÖstergötlands Läns Landsting,Linköpings universitet,Klinisk kemi,Hälsouniversitetet (författare)
Isozyme profile and tissue-origin of alkaline phosphatases in mouse serum
- Artikel/kapitelEngelska2013
Förlag, utgivningsår, omfång ...
- Elsevier,2013
- printrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:liu-90744
- https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-90744URI
- https://doi.org/10.1016/j.bone.2012.12.048DOI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
Ingår i deldatabas
- SwePubSwePub
Klassifikation
Anmärkningar
- Funding Agencies|County Council of Ostergotland in Sweden||National Institutes of Health, USA|DE012889|
- Mouse serum alkaline phosphatase (ALP) is frequently measured and interpreted in mammalian bone research. However, little is known about the circulating ALPs in mice and their relation to human ALP isozymes and isoforms. Mouse ALP was extracted from liver, kidney, intestine, and bone from vertebra, femur and calvaria tissues. Serum from mixed strains of wild-type (WT) mice and from individual ALP knockout strains were investigated, i.e., Alpl(-/-) (a.k.a. Akp2 encoding tissue-nonspecific ALP or TNALP), Akp3(-/-) (encoding duodenum-specific intestinal ALP or dIALP), and Alpi(-/-) (a.k.a. Akp6 encoding global intestinal ALP or gIALP). The ALP isozymes and isoforms were identified by various techniques and quantified by high-performance liquid chromatography. Results from the WT and knockout mouse models revealed identical bone-specific ALP isoforms (B/I. B1, and B2) as found in human serum, but in addition mouse serum contains the B1x isoform only detected earlier in patients with chronic kidney disease and in human bone tissue. The two murine intestinal isozymes, dIALP and gIALP, were also identified in mouse serum. All four bone-specific ALP isoforms (B/I, B1x, B1, and B2) were identified in mouse bones, in good correspondence with those found in human bones. All mouse tissues, except liver and colon, contained significant ALP activities. This is a notable difference as human liver contains vast amounts of ALP. Histochemical staining, Northern and Western blot analyses confirmed undetectable ALP expression in liver tissue. ALP activity staining showed some positive staining in the bile canaliculi for BALB/c and FVB/N WT mice, but not in C57BI/6 and ICR mice. Taken together, while the main source of ALP in human serum originates from bone and liver, and a small fraction from intestine (andlt;5%), mouse serum consists mostly of bone ALP, including all four isoforms, B/I, B1x, B1, and B2, and two intestinal ALP isozymes dIALP and gIALR We suggest that the genetic nomenclature for the Alpl gene in mice (i.e., ALP liver) should be reconsidered since murine liver has undetectable amounts of ALP activity. These findings should pave the way for the development of user-friendly assays measuring circulating bone-specific ALP in mouse models used in bone and mineral research.
Ämnesord och genrebeteckningar
- Alkaline phosphatase
- Bone
- Glycosylation
- Hypophosphatasia
- Knockout mice
- Mineralization
- MEDICINE
- MEDICIN
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Englund, UlrikaLinköpings universitet,Cellbiologi,Hälsouniversitetet(Swepub:liu)ulren45 (författare)
- Narisawa, SonokoSanford Burnham Medical Research Institute, CA USA (författare)
- Luis Millan, JoseSanford Burnham Medical Research Institute, CA USA (författare)
- Magnusson, PerÖstergötlands Läns Landsting,Linköpings universitet,Klinisk kemi,Hälsouniversitetet(Swepub:liu)perma28 (författare)
- Linköpings universitetKlinisk kemi (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Bone: Elsevier53:2, s. 399-4088756-32821873-2763
Internetlänk
- 1 av 1
- Föregående post
- Nästa post
- Till träfflistan
Hitta mer i SwePub
- Av författaren/redakt...
- Linder, Cecilia
- Englund, Ulrika
- Narisawa, Sonoko
- Luis Millan, Jos ...
- Magnusson, Per
- Artiklar i publikationen
- Bone
- Av lärosätet
- Linköpings universitet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
