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Epoxy Cross-Linked ...
Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes
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- Koh, Li Buay (författare)
- Linköpings universitet,Sensorvetenskap och Molekylfysik,Tekniska högskolan
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- Islam, Mohammad Mirazul (författare)
- Karolinska Institutet,Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet,Swedish Nanoscience Center, Karolinska Institute, Stockholm , Sweden
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- Mitra, Debbie (författare)
- Ottawa Hospital Research Institute, University of Ottawa Eye Institute, ON, Canada
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- Noel, Christopher (författare)
- Ottawa Hospital Research Institute, University of Ottawa Eye Institute, ON, Canada
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- Merett, Kimberley (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet,Ottawa Hospital Research Institute, University of Ottawa Eye Institute, ON, Canada
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- Odorcic, Silvia (författare)
- Ottawa Hospital Research Institute, University of Ottawa Eye Institute, ON, Canada
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- Fagerholm, Per (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Avdelningen för neurovetenskap,Hälsouniversitetet,Ögonkliniken US/LiM
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- Jackson, William Bruce (författare)
- Ottawa Hospital Research Institute, University of Ottawa Eye Institute, ON, Canada
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- Liedberg, Bo (författare)
- Center for Biomimetic Sensor Science, Nanyang Technological University, Singapore
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- Phopase, Jaywant (författare)
- Linköpings universitet,Sensorvetenskap och Molekylfysik,Tekniska högskolan
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- Griffith, May (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet,Swedish Nanoscience Center, Karolinska Institute, Stockholm, Sweden
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(creator_code:org_t)
- 2013-08-28
- 2013
- Engelska.
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Ingår i: Journal of Functional Biomaterials. - Basel, Switzerland : MDPI AG. - 2079-4983. ; 4:3, s. 162-177
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Abstract
Ämnesord
Stäng
- A bi-functional epoxy-based cross-linker, 1,4-Butanediol diglycidyl ether (BDDGE), was investigated in the fabrication of collagen based corneal substitutes. Two synthetic strategies were explored in the preparation of the cross-linked collagen scaffolds. The lysine residues of Type 1 porcine collagen were directly cross-linked using l,4-Butanediol diglycidyl ether (BDDGE) under basic conditions at pH 11. Alternatively, under conventional methodology, using both BDDGE and 1-Ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) as cross-linkers, hydrogels were fabricated under acidic conditions. In this latter strategy, Cu(BF4)2·XH2O was used to catalyze the formation of secondary amine bonds. To date, we have demonstrated that both methods of chemical cross-linking improved the elasticity and tensile strength of the collagen implants. Differential scanning calorimetry and biocompatibility studies indicate comparable, and in some cases, enhanced properties compared to that of the EDC/NHS controls. In vitro studies showed that human corneal epithelial cells and neuronal progenitor cell lines proliferated on these hydrogels. In addition, improvement of cell proliferation on the surfaces of the materials was observed when neurite promoting laminin epitope, IKVAV, and adhesion peptide, YIGSR, were incorporated. However, the elasticity decreased with peptide incorporation and will require further optimization. Nevertheless, we have shown that epoxy cross-linkers should be further explored in the fabrication of collagen-based hydrogels, as alternatives to or in conjunction with carbodiimide cross-linkers.
Nyckelord
- biomimetic materials; cross-linking; collagen; cornea; tissue engineering
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- art (ämneskategori)
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- Av författaren/redakt...
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Koh, Li Buay
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Islam, Mohammad ...
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Mitra, Debbie
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Noel, Christophe ...
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Merett, Kimberle ...
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Odorcic, Silvia
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visa fler...
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Fagerholm, Per
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Jackson, William ...
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Liedberg, Bo
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Phopase, Jaywant
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Griffith, May
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- Artiklar i publikationen
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Journal of Funct ...
- Av lärosätet
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Linköpings universitet
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Karolinska Institutet