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  • Madeja, ZbigniewKarolinska Institutet, Karolinska University Hospital in Huddinge, Stockholm, Sweden / Jagiellonian University, Kraków, Poland (författare)

The role of thioredoxin reductase activity in selenium-induced cytotoxicity

  • Artikel/kapitelEngelska2005

Förlag, utgivningsår, omfång ...

  • Elsevier,2005
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:liu-98709
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-98709URI
  • https://doi.org/10.1016/j.bcp.2005.02.023DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:1961869URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • The selenoprotein thioredoxin reductase is a key enzyme in selenium metabolism, reducing selenium compounds and thereby providing selenide to synthesis of all selenoproteins. We evaluated the importance of active TrxR1 in selenium-induced cytotoxicity using transfected TrxR1 over-expressing stable Human Embryo Kidney (HEK-293) cells and modulation of activity by pretreatment with low concentration of selenite. Treatment with sodium selenite induced cytotoxity in a dose-dependent manner in both TrxR1 over-expressing and control cells. However, TrxR1 over-expressing cells, which were preincubated for 72h with 0.1 microM selenite, were significantly more resistant to selenite cytotoxicity than control cells. To demonstrate the early effects of selenite on behaviour of HEK-293 cells, we also investigated the influence of this compound on cell motility. We observed inhibition of cell motility by 50 microM selenite immediately after administration. Moreover, TrxR1 over-expressing cells preincubated with a low concentration of selenite were more resistant to the inhibitory effect of 50 microM selenite than those not preincubated. It was also observed that the TrxR over-expressing cells showed higher TrxR1 activity than control cells and the preincubation of over-expressing cells with 0.1 microM selenite induced further significant increase in the activity of TrxR1. On the other hand, we demonstrated that TrxR1 over-expressing cells showed decreased glutathione peroxidase activity compared to control cells. These data strongly suggest that TrxR1 may be a crucial enzyme responsible for cell resistance against selenium cytotoxicity.

Ämnesord och genrebeteckningar

  • selenium; thioredoxin reductase; cytotoxicity; glutathione peroxidase; cell motility; oxidative stress

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Sroka, JolantaKarolinska Institutet, Karolinska University Hospital in Huddinge, Stockholm, Sweden / Jagiellonian University, Kraków, Poland (författare)
  • Nyström, ChristinaKarolinska Institutet, Karolinska University Hospital in Huddinge, Stockholm, Sweden (författare)
  • Björkhem-Bergman, LindaKarolinska Institutet (författare)
  • Nordman, TomasKarolinska Institutet, Karolinska University Hospital in Huddinge, Stockholm, Sweden (författare)
  • Damdimopoulos, AnastasiosKarolinska Institutet (författare)
  • Nalvarte, IvanKarolinska Institutet (författare)
  • Eriksson, Lennart C.Karolinska Institutet (författare)
  • Spyrou, GiannisFoundation of Biomedical Research, Academy of Athens, Greece / Karolinska Institutet, Huddinge, Sweden(Swepub:liu)ioasp42 (författare)
  • Olsson, Jerker M.Karolinska Institutet, Karolinska University Hospital in Huddinge, Stockholm, Sweden (författare)
  • Björnstedt, MikaelKarolinska Institutet (författare)
  • Karolinska Institutet, Karolinska University Hospital in Huddinge, Stockholm, Sweden / Jagiellonian University, Kraków, PolandKarolinska Institutet, Karolinska University Hospital in Huddinge, Stockholm, Sweden (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Biochemical Pharmacology: Elsevier69:12, s. 1765-17720006-29521356-1839

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