Sökning: WFRF:(Takabayashi A. Y.) >
Possible involvemen...
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Sasada, T.Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto, Japan
(författare)
Possible involvement of thioredoxin reductase as well as thioredoxin in cellular sensitivity to cis-diamminedichloroplatinum (II)
- Artikel/kapitelEngelska1999
Förlag, utgivningsår, omfång ...
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Elsevier,1999
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-98827
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-98827URI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1944032URI
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https://doi.org/10.1016/S0891-5849(99)00101-XDOI
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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The thioredoxin (TRX) system, composed of nicotinamide adenine dinucleotide phosphate (reduced form), TRX, and TRX reductase (TRXR), has multiple biologic functions via thiol-mediated redox control. In this study, we investigated the relationship between intracellular TRXR levels and cellular sensitivity to cis-diamminedichloroplatinum (II) (CDDP). HeLa, a human cervical carcinoma cell line, cultured with CDDP showed a time- and dose-dependent reduction of intracellular TRXR activity, which was well correlated with the decrease in cell viability after exposure to CDDP. In a cell-free system, CDDP was found to directly inactivate the reduced form of purified human TRXR. The CDDP-resistant variants of HeLa cells, established by continuous exposure to CDDP, exhibited an increased expression and activity of TRXR as well as TRX compared with the parental cells. In addition, sodium selenate, an inhibitor of TRXR, was found to increase the susceptibility to CDDP in the CDDP-resistant cells. Moreover, the HeLa cells transfected with an antisense TRXR RNA expression vector to reduce the intracellular enzyme activity displayed an enhanced sensitivity to CDDP. Taken together with previous reports on TRX, these results indicate the possible involvement of TRXR as well as TRX in the cellular sensitivity and resistance to CDDP.
Ämnesord och genrebeteckningar
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Thioredoxin
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Thioredoxin reductase
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cis-Diamminedichloroplatinum (II)
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Cytotoxicity
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Redox
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Free radicals
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Nakamura, H.Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto, Japan
(författare)
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Ueda, S.Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto, Japan
(författare)
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Sato, N.Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto, Japan
(författare)
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Kitaoka, Y.Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto, Japan
(författare)
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Gon, Y.Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto, Japan
(författare)
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Takabayashi, A.Department of Surgery, Tazuke Kofukai Kitano Hospital Medical Institute, Osaka, Japan
(författare)
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Spyrou, GiannisMedical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden(Swepub:liu)ioasp42
(författare)
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Holmgren, ArneKarolinska Institutet
(författare)
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Yodoi, J.Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto, Japan
(författare)
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Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto, JapanDepartment of Surgery, Tazuke Kofukai Kitano Hospital Medical Institute, Osaka, Japan
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Free Radical Biology & Medicine: Elsevier27:5-6, s. 504-5140891-58491873-4596
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Till lärosätets databas
- Av författaren/redakt...
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Sasada, T.
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Nakamura, H.
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Ueda, S.
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Sato, N.
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Kitaoka, Y.
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Gon, Y.
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visa fler...
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Takabayashi, A.
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Spyrou, Giannis
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Holmgren, Arne
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Yodoi, J.
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visa färre...
- Artiklar i publikationen
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Free Radical Bio ...
- Av lärosätet
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Linköpings universitet
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Karolinska Institutet