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Characterization of...
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Appelkvist, Eewa-LiisaKarolinska Institutet, Huddinge Hospital, Sweden
(author)
Characterization of the lipid and protein contents of myelin bodies isolated from the renal cortex of gentamicin-treated rats
- Article/chapterEnglish1991
Publisher, publication year, extent ...
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Elsevier,1991
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:liu-99876
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-99876URI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Myelin bodies were isolated from the renal cortex of gentamicin-treated rats (100 mg/kg body weight, twice daily for 3 days, i.p.) employing an initial pelleting by differential centrifugation and subsequent flotation on a discontinuous sucrose gradient. These structures were found to contain almost twice as much protein as phospholipid and SDS-polyacrylamide gel electrophoresis revealed the presence of many different polypeptides. All the major phospholipids are present, although myelin bodies contain a considerably higher proportion of phosphatidylinositol, somewhat more phosphatidylcholine and considerably lower percentages of phosphatidylserine and sphingomyelin than do normal renal phospholipids. The fatty acids of myelin body phospholipids are highly saturated (67.3-87.9%) and a striking feature is the occurrence of relatively large amounts of 22:1, presumably erucic acid, especially in sphingomyelin. Myelin bodies contain small amounts of unesterified cholesterol, unesterified dolichol and coenzymes Q9 and Q10.
Added entries (persons, corporate bodies, meetings, titles ...)
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Söderström, MatsArrhenius Laboratory for the Natural Sciences, Stockholm University, Sweden(Swepub:liu)matso48
(author)
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Nässberger, LennartStockholm University, Sweden
(author)
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Damberg, CharlottaStockholm University, Sweden
(author)
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Dallner, GustavKarolinska Institutet, Huddinge Hospital, Sweden / Stockholm University, Sweden
(author)
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DePierre, Joseph WStockholm University, Sweden
(author)
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Karolinska Institutet, Huddinge Hospital, SwedenArrhenius Laboratory for the Natural Sciences, Stockholm University, Sweden
(creator_code:org_t)
Related titles
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In:Biochemical and Biophysical Research Communications - BBRC: Elsevier181:2, s. 894-9010006-291X1090-2104
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