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In vitro phosphoryl...
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Nilsson Ekdahl, KristinaDepartment of Medical and Physiological Chemistry, University of Uppsala
(författare)
In vitro phosphorylation of fructose-1,6-bisphosphatase from rabbit and pig liver with cyclic AMP-dependent protein kinase
- Artikel/kapitelEngelska1988
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Elsevier BV,1988
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LIBRIS-ID:oai:DiVA.org:lnu-1106
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https://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-1106URI
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https://doi.org/10.1016/0003-9861(88)90164-6DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Homogeneous preparations of fructose-1,6-bisphosphatase from mouse, man, rabbit, pig, and rat were tested as substrates for cyclic AMP-dependent protein kinase. Up to 1 mol of [32P]phosphate per mole enzyme subunit was incorporated into fructose-1,6-bisphosphatase from pig and rabbit liver, which should be compared with 2.6 mol of phosphate per mole enzyme subunit in the case of the rat liver enzyme. The phosphorylation of fructose-1,6-bisphosphatase from the livers of man and mouse was negligible. Phosphorylation of pig and rabbit fructose-1,6-bisphosphatase decreased the apparent Km for fructose-1,6-bisphosphate, but in contrast to the case of the rat liver enzyme it did not change the inhibition constants for AMP and fructose-2,6-bisphosphate. The Phosphorylation sites in rabbit and pig liver fructose-1,6-bisphosphatase were located close to the carboxyterminal of the polypeptide chains, since trypsin treatment of the phosphorylated enzyme quantitatively removed all of the protein-bound radioactivity without significantly altering the subunit molecular weight and with a maintained neutral pH Optimum.
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Department of Medical and Physiological Chemistry, University of Uppsala
(creator_code:org_t)
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Ingår i:Archives of Biochemistry and Biophysics: Elsevier BV262:1, s. 27-310003-98611096-0384
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