Thrombin Differentially Modulates the Acute Inflammatory Response to Escherichia coli and Staphylococcus aureus in Human Whole Blood

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: (WFRF:(Woodruff Trent M.)) > Thrombin Differenti...

Details
MARC

Johnson, ChristinaUniv Oslo, Norway;RIKEN Oslo Univ Hosp, Norway (author)

Thrombin Differentially Modulates the Acute Inflammatory Response to Escherichia coli and Staphylococcus aureus in Human Whole Blood

Article/chapterEnglish2022

Publisher, publication year, extent ...

2022-06-15
American Association of Immunologists :AMER ASSOC IMMUNOLOGISTS,2022
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:lnu-115623
https://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-115623URI
https://doi.org/10.4049/jimmunol.2101033DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Thrombin plays a central role in thromboinflammatory responses, but its activity is blocked in the common ex vivo human whole blood models, making an ex vivo study of thrombin effects on thromboinflammatory responses unfeasible. In this study, we exploited the anticoagulant peptide Gly-Pro-Arg-Pro (GPRP) that blocks fibrin polymerization to study the effects of thrombin on acute inflammation in response to Escherichia coli and Staphylococcus aureus. Human blood was anticoagulated with either GPRP or the thrombin inhibitor lepirudin and incubated with either E. coli or S. aureus for up to 4 h at 37 degrees C. In GPRP-anticoagulated blood, there were spontaneous elevations in thrombin levels and platelet activation, which further increased in the presence of bacteria. Complement activation and the expression of activation markers on monocytes and granulocytes increased to the same extent in both blood models in response to bacteria. Most cytokines were not elevated in response to thrombin alone, but thrombin presence substantially and heterogeneously modulated several cytokines that increased in response to bacterial incubations. Bacterial-induced releases of IL-8, MIP-1 alpha, and mip-1 beta were potentiated in the thrombin-active GPRP model, whereas the levels of IP-10, TNF, IL-6, and IL-1 beta were elevated in the thrombin-inactive lepirudin model. Complement CS-blockade, combined with CD14 inhibition, reduced the overall cytokine release significantly, both in thrombin-active and thrombin-inactive models. Our data support that thrombin itself marginally induces leukocyte-dependent cytokine release in this isolated human whole blood but is a significant modulator of bacteria-induced inflammation by a differential effect on cytokine patterns.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Immunologi inom det medicinska området hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Immunology in the medical area hsv//eng
Immunologi
Immunology

Added entries (persons, corporate bodies, meetings, titles ...)

Quach, Huy QuangUniv Oslo, Norway;RIKEN Oslo Univ Hosp, Norway (author)
Lau, CorinnaNordland Hosp, Norway (author)
Ekholt, KarinUniv Oslo, Norway;RIKEN Oslo Univ Hosp, Norway (author)
Espevik, TerjeNorwegian Univ Sci & Technol, Norway (author)
Woodruff, Trent M.Univ Queensland, Australia (author)
Pischke, Soren ErikUniv Oslo, Norway;RIKEN Oslo Univ Hosp, Norway (author)
Mollnes, Tom EirikUniv Oslo, Norway;RIKEN Oslo Univ Hosp, Norway;Nordland Hosp, Norway;Norwegian Univ Sci & Technol, Norway;Univ Tromso, Norway (author)
Nilsson, Per H.,1980-Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Avancerade material,Univ Oslo, Norway;RIKEN Oslo Univ Hosp, Norway,Linnaeus Ctr Biomat Chem, BMC;HoRB(Swepub:lnu)enipe (author)
Univ Oslo, Norway;RIKEN Oslo Univ Hosp, NorwayNordland Hosp, Norway (creator_code:org_t)

Related titles

In:Journal of ImmunologyAmerican Association of Immunologists : AMER ASSOC IMMUNOLOGISTS208:12, s. 2771-27780022-17671550-6606

Internet link

Find in a library

Journal of Immunology (Search for host publication in LIBRIS)

To the university's database

Find more in SwePub

By the author/editor: Johnson, Christi ...; Quach, Huy Quang; Lau, Corinna; Ekholt, Karin; Espevik, Terje; Woodruff, Trent ...; show more...; Pischke, Soren E ...; Mollnes, Tom Eir ...; Nilsson, Per H., ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Immunology in th ...

Articles in the publication: Journal of Immun ...

By the university: Linnaeus University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se