A cell glue : Inducing cell adhesion using surface modification with cell-penetrating peptide-peg-lipid for 3d cell structures

• Statement of Purpose: The ultimate goal of regenerative therapy is the transplantation of functional stem cells-derived tissues and organs to replace those lost as the result of pathology or tissue damage. Since tissues and organs are complicated 3D structures, 3D scaffolds such as decellularized organs and tissues, are required to properly orient living functional cells of different types. 3D scaffolds offer an environment for cell adhesion that differs from that of conventional 2D culture. Therefore, the induction and control of cell attachment, not only to 2D substrate surfaces but also to 3D scaffolds, is of great importance. Here, we propose new type of cell glue made of cell-penetrating peptides (CPP) and PEG-conjugated lipid, which are used for cell-surface modification. PEG-lipid derivatives are incorporated into the lipid bilayer membranes of cells via hydrophobic interactions, and the CPP anchored onto the cell membrane could work as an adhesive domain. In our study, various floating cells, (i.e., T cells, B cells) were used to examine the adhesive efficacy by cell surface modification with CPP-PEG-lipid onto material surface as well as PS microfiber-based 3D scaffolds.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

cell surface modification

poly(ethylene glycol)-conjugated phospholipid (PEG-lipid)

cell-penetrating peptide (CPP)

cell adhesion

3D structure

Biomedical Sciences

Biomedicinsk vetenskap

Publikations- och innehållstyp

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