Inflamed In Vitro Retina: Cytotoxic Neuroinflammation and Galectin-3 Expression

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: WFRF:(Johansson Patrik) > (2015-2019) > Inflamed In Vitro R...

Details
MARC

Bauer, Patrik MaximilianLund University,Lunds universitet,Oftalmologi (Malmö),Forskargrupper vid Lunds universitet,Biologiska institutionen,Naturvetenskapliga fakulteten,Ophthalmology (Malmö),Lund University Research Groups,Department of Biology,Faculty of Science,Lund University, Sweden (author)

Inflamed In Vitro Retina : Cytotoxic Neuroinflammation and Galectin-3 Expression

Article/chapterEnglish2016

Publisher, publication year, extent ...

2016-09-09
Public Library of Science (PLoS),2016
electronicrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:lnu-120688
https://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-120688URI
https://doi.org/10.1371/journal.pone.0161723DOI
https://lup.lub.lu.se/record/cdc0321c-7449-44e5-8ab7-db4097322d29URI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Background Disease progression in retinal neurodegeneration is strongly correlated to immune cell activation, which may have either a neuroprotective or neurotoxic effect. Increased knowledge about the immune response profile and retinal neurodegeneration may lead to candidate targets for treatments. Therefore, we have used the explanted retina as a model to explore the immune response and expression of the immune modulator galectin-3 (Gal-3), induced by the cultivation per se and after additional immune stimulation with lipopolysaccharide (LPS), and how this correlates with retinal neurotoxicity. Methods Post-natal mouse retinas were cultured in a defined medium. One group was stimulated with LPS (100 ng/ml, 24 h). Retinal architecture, apoptotic cell death, and micro- and macroglial activity were studied at the time of cultivation (0 days in vitro (DIV)) and at 3, 4 and 7 DIV using morphological staining, biochemical- and immunohistochemical techniques. Results Our results show that sustained activation of macro- and microglia, characterized by no detectable cytokine release and limited expression of Gal-3, is not furtherinducing apoptosis additional to the axotomy-induced apoptosis in innermost nuclear layer. An elevated immune response was detected after LPS stimulation, as demonstrated primarily by release of immune mediators (i.e. interleukin 2 (IL-2), IL-6, KC/GRO (also known as CLCX1) and tumour necrosis factor-α (TNF-α)), increased numbers of microglia displaying morphologies of late activation stages as well as Gal-3 expression. This was accompanied with increased apoptosis in the two additional nuclear layers, and damage to retinal gross architecture. Conclusion We demonstrate that an immune response characterized by sustained and increased release of cytokines, along with an increase in Gal-3 expression, is accompanied by significant increased neurotoxicity in the explanted retina. Further investigations using the current setting may lead to increased understanding on the mechanisms involved in neuronal loss in retinal neurodegenerations. © 2016 Bauer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Oftalmologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Ophthalmology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Immunologi inom det medicinska området hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Immunology in the medical area hsv//eng
Biomedical Sciences
Biomedicinsk vetenskap

Added entries (persons, corporate bodies, meetings, titles ...)

Zalis, Marina CastroLund University,Lunds universitet,Oftalmologi, Lund,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Ophthalmology, Lund,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University, Sweden(Swepub:lu)med-mcz (author)
Abdshill, HodanLund University,Lunds universitet,Oftalmologi, Lund,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Ophthalmology, Lund,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University, Sweden(Swepub:lu)nkir-hab (author)
Deierborg, TomasLund University,Lunds universitet,Kansli för experimentell medicinsk vetenskap,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department Office of Experimental Medical Science,Department of Experimental Medical Science,Faculty of Medicine,Lund University, Sweden(Swepub:lu)expb-tol (author)
Johansson, FredrikLund University,Lunds universitet,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science,Lund University, Sweden(Swepub:lu)cob-fjs (author)
Englund Johansson, UlricaLund University,Lunds universitet,Oftalmologi, Lund,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Ophthalmology, Lund,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University, Sweden(Swepub:lu)mphy-uen (author)
Oftalmologi (Malmö)Forskargrupper vid Lunds universitet (creator_code:org_t)

Related titles

In:PLOS ONE: Public Library of Science (PLoS)11:91932-6203

Internet link

Find in a library

PLOS ONE (Search for host publication in LIBRIS)

To the university's database

Find more in SwePub

By the author/editor: Bauer, Patrik Ma ...; Zalis, Marina Ca ...; Abdshill, Hodan; Deierborg, Tomas; Johansson, Fredr ...; Englund Johansso ...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Ophthalmology

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Immunology in th ...

Articles in the publication: PLOS ONE

By the university: Linnaeus University; Lund University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se