Sökning: WFRF:(Mollnes Tom Eirik) >
Escherichia coli-in...
Escherichia coli-induced inflammatory responses are temperature-dependent in human whole blood ex vivo
-
- Chaban, Viktoriia (författare)
- Univ Oslo, Norway;Oslo Univ Hosp, Norway
-
- de Boer, Eline (författare)
- Univ Oslo, Norway;Oslo Univ Hosp, Norway
-
- McAdam, Karin E. (författare)
- Univ Oslo, Norway;Oslo Univ Hosp, Norway
-
visa fler...
-
- Vaage, Jarle (författare)
- Univ Oslo, Norway;Oslo Univ Hosp, Norway
-
- Mollnes, Tom Eirik (författare)
- Univ Oslo, Norway;Oslo Univ Hosp, Norway;Nordland Hosp, Norway;Norwegian Univ Sci & Technol, Norway
-
- Nilsson, Per H., 1980- (författare)
- Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Oslo Univ Hosp, Norway,Linnaeus Ctr Biomat Chem, BMC;HoRB
-
- Piscike, Soren Erik (författare)
- Univ Oslo, Norway;Oslo Univ Hosp, Norway
-
- Islam, Rakibul (författare)
- Univ Oslo, Norway;Oslo Univ Hosp, Norway
-
visa färre...
-
(creator_code:org_t)
- Elsevier, 2023
- 2023
- Engelska.
-
Ingår i: Molecular Immunology. - : Elsevier. - 0161-5890 .- 1872-9142. ; 157, s. 70-77
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Systemic inflammatory conditions are often associated with hypothermia or hyperthermia. Therapeutic hypothermia is used in post-cardiac arrest and some other acute diseases. There is a need for more knowledge concerning the effect of various temperatures on the acute inflammatory response. The complement system plays a crucial role in initiating the inflammatory response. We hypothesized that temperatures above and below the physiologic 37 & DEG;C affect complement activation and cytokine production ex vivo. Lepirudin-anticoagulated human whole blood from 10 healthy donors was incubated in the presence or absence of Escherichia coli at different temperatures (4 & DEG;C, 12 & DEG;C, 20 & DEG;C, 33 & DEG;C, 37 & DEG;C, 39 & DEG;C, and 41 & DEG;C). Complement activation was assessed by the terminal C5b-9 complement complex (TCC) and the alternative convertase C3bBbP using ELISA. Cytokines were measured using a 27-plex assay. Granulocyte and monocyte activation was evaluated by CD11b surface expression using flow cytometry. A consistent increase in complement activation was observed with rising temperature, reaching a maximum at 41 & DEG;C, both in the absence (C3bBbP p < 0.05) and presence (C3bBbP p < 0.05 and TCC p < 0.05) of E. coli. Temperature alone did not affect cytokine production, whereas incubation with E. coli significantly increased cytokine levels of IL-18, IL-2, IL-6, IL-8, IFN-& gamma;, and TNF at temperatures > 20 & DEG;C. Maximum increase occurred at 39 & DEG;C. However, a consistent decrease was observed at 41 & DEG;C, significant for IL-18 (p = 0.003). Granulocyte CD11b displayed the same temperature-dependent pattern as cytokines, with a corresponding increase in endothelial cell apoptosis and necrosis. Thus, blood temperature differentially determines the degree of complement activation and cytokine release.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Comblement system
- Innate immunity
- Cytokine
- Temperature
- Hypothermia
- Hyperthermia
- Immunologi
- Immunology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas