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Exploitation of Eng...
Exploitation of Engineered Light-Switchable Myosin XI for Nanotechnological Applications
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- Salhotra, Aseem (författare)
- Linnaeus University,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Lund University, Sweden
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- Rahman, Mohammad A. (författare)
- Linnaeus University,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB)
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- Ruijgrok, Paul, V (författare)
- Stanford Univ, USA,Stanford University
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- Meinecke, Christoph R. (författare)
- Tech Univ Chemnitz, Germany,Chemnitz University of Technology
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- Ušaj, Marko, Research Engineer (författare)
- Linnaeus University,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Lund University, Sweden
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- Zemsky, Sasha (författare)
- Stanford Univ, USA,Stanford University
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- Lindberg, Frida W. (författare)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
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- Surendiran, Pradheebha (författare)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,LTH profilområde: Nanovetenskap och halvledarteknologi,LTH profilområden,LU profilområde: Ljus och material,Lunds universitets profilområden,Other operations, LTH,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH,LTH Profile Area: Nanoscience and Semiconductor Technology,LTH Profile areas,Faculty of Engineering, LTH,LU Profile Area: Light and Materials,Lund University Profile areas
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- Lyttleton, Roman W. (författare)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,LTH profilområde: Nanovetenskap och halvledarteknologi,LTH profilområden,LU profilområde: Ljus och material,Lunds universitets profilområden,Other operations, LTH,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH,LTH Profile Area: Nanoscience and Semiconductor Technology,LTH Profile areas,Faculty of Engineering, LTH,LU Profile Area: Light and Materials,Lund University Profile areas
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- Linke, Heiner (författare)
- Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,LTH profilområde: Nanovetenskap och halvledarteknologi,LTH profilområden,LTH profilområde: Teknik för hälsa,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH,LTH Profile Area: Nanoscience and Semiconductor Technology,LTH Profile areas,Faculty of Engineering, LTH,LTH Profile Area: Engineering Health,Faculty of Engineering, LTH
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- Korten, Till (författare)
- Tech Univ Dresden, Germany,Dresden University of Technology
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- Bryant, Zev (författare)
- Stanford Univ, USA,Stanford University
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- Månsson, Alf (författare)
- Linnaeus University,Lunds universitet,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Lund University, Sweden,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Other operations, LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- American Chemical Society (ACS), 2023
- 2023
- Engelska.
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Ingår i: ACS Nano. - : American Chemical Society (ACS). - 1936-0851 .- 1936-086X. ; 17:17, s. 17233-17244
- Relaterad länk:
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https://doi.org/10.1...
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http://dx.doi.org/10... (free)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- For certain nanotechnological applications of the contractile proteins actin and myosin, e.g., in biosensing and network-based biocomputation, it would be desirable to temporarily switch on/off motile function in parts of nanostructured devices, e.g., for sorting or programming. Myosin XI motor constructs, engineered with a light-switchable domain for switching actin motility between high and low velocities (light-sensitive motors (LSMs) below), are promising in this regard. However, they were not designed for use in nanotechnology, where longevity of operation, long shelf life, and selectivity of function in specific regions of a nanofabricated network are important. Here, we tested if these criteria can be fulfilled using existing LSM constructs or if additional developments will be required. We demonstrated extended shelf life as well as longevity of the actin-propelling function compared to those in previous studies. We also evaluated several approaches for selective immobilization with a maintained actin propelling function in dedicated nanochannels only. Whereas selectivity was feasible using certain nanopatterning combinations, the reproducibility was not satisfactory. In summary, the study demonstrates the feasibility of using engineered light-controlled myosin XI motors for myosin-driven actin transport in nanotechnological applications. Before use for, e.g., sorting or programming, additional work is however needed to achieve reproducibility of the nanofabrication and, further, optimize the motor properties.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
Nyckelord
- engineered myosin XI
- actin
- light-switchablemotor
- nanofabrication
- surface chemistry
- spatiotemporal motility control
- Biokemi
- Biochemistry
- actin
- engineered myosin XI
- light-switchable motor
- nanofabrication
- spatiotemporal motility control
- surface chemistry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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ACS Nano
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Till lärosätets databas
- Av författaren/redakt...
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Salhotra, Aseem
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Rahman, Mohammad ...
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Ruijgrok, Paul, ...
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Meinecke, Christ ...
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Ušaj, Marko, Res ...
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Zemsky, Sasha
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visa fler...
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Lindberg, Frida ...
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Surendiran, Prad ...
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Lyttleton, Roman ...
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Linke, Heiner
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Korten, Till
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Bryant, Zev
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Månsson, Alf
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biofysik
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Materialkemi
- Artiklar i publikationen
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ACS Nano
- Av lärosätet
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Linnéuniversitetet
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Lunds universitet