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Storage of Transfus...
Storage of Transfusion Platelet Concentrates is Associated with Complement Activation and Reduced Ability of Platelets to Respond to Protease-Activated Receptor-1 and Thromboxane A2 Receptor
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- Andersson, Linnea (author)
- Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Linnaeus Univ, Sweden
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- Sjöström, Dick J. (author)
- Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Linnaeus Univ, Sweden
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- Quach, Huy Quang (author)
- Mayo Clin, MN 55905 USA
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- Hägerström, Kim (author)
- Region Kalmar County, Sweden,Dept Clin Chem & Transfus Med, Sweden
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- Hurler, Lisa (author)
- Semmelwe Univ, Hungary
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- Kajdacsi, Erika (author)
- Semmelwe Univ, Hungary
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- Cervenak, Laszlo (author)
- Semmelwe Univ, Hungary
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- Prohaszka, Zoltan (author)
- Semmelwe Univ, Hungary
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- Toonen, Erik J. M. (author)
- Hycult Biotechnology, Netherlands,R&D Dept, Netherlands
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- Mohlin, Camilla, 1972- (author)
- Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Linnaeus Univ, Sweden
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- Mollnes, Tom Eirik (author)
- Univ Oslo, Norway;Oslo Univ Hosp, Norway;Nordland Hosp, Norway
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- Sandgren, Per (author)
- Karolinska Institutet, Sweden,Karolinska Inst, Sweden
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- Tjernberg, Ivar (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Dept Clin Chem & Transfus Med, Sweden
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- Nilsson, Per H., 1980- (author)
- Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Linnaeus Ctr Biomat Chem, BMC,Linnaeus Univ, Sweden; Linnaeus Univ, Sweden
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(creator_code:org_t)
- MDPI, 2024
- 2024
- English.
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In: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 25:2
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Abstract
Subject headings
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- Platelet activation and the complement system are mutually dependent. Here, we investigated the effects of storage time on complement activation and platelet function in routinely produced platelet concentrates. The platelet concentrates (n = 10) were stored at 22 degrees C for seven days and assessed daily for complement and platelet activation markers. Additionally, platelet function was analyzed in terms of their responsiveness to protease-activated receptor-1 (PAR-1) and thromboxane A2 receptor (TXA(2)R) activation and their capacity to adhere to collagen. Complement activation increased over the storage period for all analyzed markers, including the C1rs/C1-INH complex (fold change (FC) = 1.9; p < 0.001), MASP-1/C1-INH complex (FC = 2.0; p < 0.001), C4c (FC = 1.8, p < 0.001), C3bc (FC = 4.0; p < 0.01), and soluble C5b-9 (FC = 1.7, p < 0.001). Furthermore, the levels of soluble platelet activation markers increased in the concentrates over the seven-day period, including neutrophil-activating peptide-2 (FC = 2.5; p < 0.0001), transforming growth factor beta 1 (FC = 1.9; p < 0.001) and platelet factor 4 (FC = 2.1; p < 0.0001). The ability of platelets to respond to activation, as measured by surface expression of CD62P and CD63, decreased by 19% and 24% (p < 0.05) for PAR-1 and 69-72% (p < 0.05) for TXA(2)R activation, respectively, on Day 7 compared to Day 1. The extent of platelet binding to collagen was not significantly impaired during storage. In conclusion, we demonstrated that complement activation increased during the storage of platelets, and this correlated with increased platelet activation and a reduced ability of the platelets to respond to, primarily, TXA(2)R activation.
Subject headings
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- platelet storage
- platelet storage lesion
- complement activation
- platelet function
- hemostasis
- Immunologi
- Immunology
- Cell and Organism Biology
- Cell- och organismbiologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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Andersson, Linne ...
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Sjöström, Dick J ...
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Quach, Huy Quang
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Hägerström, Kim
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Hurler, Lisa
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Kajdacsi, Erika
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Cervenak, Laszlo
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Prohaszka, Zolta ...
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Toonen, Erik J. ...
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Mohlin, Camilla, ...
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Mollnes, Tom Eir ...
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Sandgren, Per
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Tjernberg, Ivar
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Nilsson, Per H., ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Immunology
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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Linnaeus University
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Linköping University
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Karolinska Institutet