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Effects of overexpr...
Effects of overexpression of AaWRKY1 on artemisinin biosynthesis in transgenic Artemisia annua plants
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- Han, Junli (författare)
- Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB)
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- Wang, Hongzhen (författare)
- Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Zhejiang Agriculture and Forestry University, Linan 311300, Zhejiang, PR China.
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- Lundgren, Anneli (författare)
- Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB)
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- Brodelius, Peter E. (författare)
- Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB)
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(creator_code:org_t)
- Elsevier BV, 2014
- 2014
- Engelska.
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Ingår i: Phytochemistry. - : Elsevier BV. - 0031-9422 .- 1873-3700. ; 102, s. 89-96
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The effective anti-malarial medicine artemisinin is costly because of the low content in Artemisia annua. Genetic engineering of A. annua is one of the most promising approaches to improve the yield of artemisinin. In this work, the transcription factor AaWRKY1, which is thought to be involved in the regulation of artemisinin biosynthesis, was cloned from A. annua var. Chongqing and overexpressed using the CaMV35S promoter or the trichome-specific CYP71AV1 promoter in stably transformed A. annua plants. The transcript level of AaWRKY1 was increased more than one hundred times under the CaMV35S promoter and about 40 times under the CYP71AV1 promoter. The overexpressed AaWRKY1 activated the transcription of CYP71AV1 and moreover the trichome-specific overexpression of AaWRKY1 improved the transcription of CYP71AV1 much more effectively than the constitutive overexpression of AaWRKY1, i.e. up to 33 times as compared to the wild-type plant. However the transcription levels of FDS, ADS, and DBR2 did not change significantly in transgenic plants. The significantly up-regulated CYP71AV1 promoted artemisinin biosynthesis, i.e. up to about 1.8 times as compared to the wild-type plant. It is demonstrated that trichome-specific overexpression of AaWRKY1 can significantly activate the transcription of CYP71AV1 and the up-regulated CYP71AV1 promotes artemisinin biosynthesis.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- AaWRKY1; Artemisia annua; Artemisinin biosynthesis; CYP71AV1 promoter; CYP71AV1; Trichome-specific overexpression
- Biochemistry
- Biokemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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