Heparin molecularly imprinted surfaces for the attenuation of complement activation in blood

Rosengren-Holmberg, Jenny P.Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Linnaeus Univ, Dept Chem & Biomed Sci, Ctr Biomat Chem, SE-39182 Kalmar, Sweden.;Swedish Natl Forens Ctr, Drugs Unit, SE-58194 Linkoping, Sweden. (författare)

Heparin-imprinted synthetic polymer surfaces with the ability to attenuate activation of both the complement and the coagulation system in whole blood were successfully produced. Imprinting was achieved using a template coated with heparin, a highly sulfated glycosaminoglycan known for its anticoagulant properties. The N,N'-diacryloylpiperazine-methacrylic acid copolymers were characterized using goniometry, AFM and XPS. The influence of the molecular imprinting process on morphology and template rebinding was demonstrated by radioligand binding assays. Surface hemocompatibility was evaluated using human whole blood without anticoagulants followed by measurement of complement activation markers C3a and sC5b-9 and platelet consumption as a surrogate coagulation activation marker. The observed low thrombogenicity of this copolymer combined with the attenuation of complement activation induced by the molecular imprint offer potential for the development of self-regulating surfaces with important potential clinical applications. We propose a mechanism for the observed phenomena based upon the recruitment of endogenous sulfated glycosaminoglycans with heparin-like activities.

Andersson, JonasUppsala universitet,Klinisk immunologi,Univ Uppsala Hosp, Sweden (författare)
Smith, James R.Univ Portsmouth, UK,Univ Portsmouth, Sch Pharm & Biomed Sci, Portsmouth PO1 2DT, Hants, England. (författare)
Alexander, CameronUniv Nottingham, UK,Univ Nottingham, Sch Pharm, Nottingham NG7 2RD, England. (författare)
Alexander, Morgan R.Univ Nottingham, UK,Univ Nottingham, Sch Pharm, Nottingham NG7 2RD, England. (författare)
Tovar, GuenterUniv Stuttgart, Inst Interfacial Engn, D-70569 Stuttgart, Germany.;Fraunhofer Inst Interfacial Engn & Biotechnol, D-70569 Stuttgart, Germany. (författare)
Nilsson Ekdahl, KristinaLinnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Linnaeus Univ, Dept Chem & Biomed Sci, Ctr Biomat Chem, SE-39182 Kalmar, Sweden.;Univ Uppsala Hosp, Clin Immunol Sect, Rudbeck Lab C5, Dept Oncol Radiol & Clin Immunol, SE-75185 Uppsala, Sweden.,Linnaeus Ctr Biomat Chem, BMC(Swepub:lnu)nnikr (författare)
Nicholls, Ian A.Uppsala universitet,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Uppsala University,BBCL;Linnaeus Ctr Biomat Chem, BMC,Institutionen för kemi - BMC,Linnaeus Univ, Dept Chem & Biomed Sci, Ctr Biomat Chem, SE-39182 Kalmar, Sweden(Swepub:uu)ianni101 (författare)
LinnéuniversitetetInstitutionen för kemi och biomedicin (KOB) (creator_code:org_t)

Ingår i:Biomaterials Science: Royal Society of Chemistry (RSC)3:8, s. 1208-12172047-48302047-4849

Av författaren/redakt...: Rosengren-Holmbe ...; Andersson, Jonas; Smith, James R.; Alexander, Camer ...; Alexander, Morga ...; Tovar, Guenter; visa fler...; Nilsson Ekdahl, ...; Nicholls, Ian A.; visa färre...

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