Microbial metagenomes from three aquifers in the Fennoscandian shield terrestrial deep biosphere reveal metabolic partitioning among populations

Wu, XiaofenLinnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS (författare)

Supplementary information available for this article at http://www.nature.com/ismej/journal/v10/n5/suppinfo/ismej2015185s1.html
Microorganisms in the terrestrial deep biosphere host up to 20% of the earth's biomass and are suggested to be sustained by the gases hydrogen and carbon dioxide. A metagenome analysis of three deep subsurface water types of contrasting age (from <20 to several thousand years) and depth (171 to 448 m) revealed phylogenetically distinct microbial community subsets that either passed or were retained by a 0.22 μm filter. Such cells of <0.22 μm would have been overlooked in previous studies relying on membrane capture. Metagenomes from the three water types were used for reconstruction of 69 distinct microbial genomes, each with >86% coverage. The populations were dominated by Proteobacteria, Candidate divisions, unclassified archaea and unclassified bacteria. The estimated genome sizes of the <0.22 μm populations were generally smaller than their phylogenetically closest relatives, suggesting that small dimensions along with a reduced genome size may be adaptations to oligotrophy. Shallow 'modern marine' water showed community members with a predominantly heterotrophic lifestyle. In contrast, the deeper, 'old saline' water adhered more closely to the current paradigm of a hydrogen-driven deep biosphere. The data were finally used to create a combined metabolic model of the deep terrestrial biosphere microbial community.

Holmfeldt, KarinLinnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS(Swepub:lnu)mhoka (författare)
Hubalek, Valerie,1981-Uppsala universitet,Limnologi,Science for Life Laboratory, SciLifeLab,Uppsala University(Swepub:uu)valhu921 (författare)
Lundin, DanielLinnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS(Swepub:lnu)daluab (författare)
Åström, Mats E.Linnéuniversitetet,Institutionen för biologi och miljö (BOM)(Swepub:lnu)kasma (författare)
Bertilsson, StefanUppsala universitet,Science for Life Laboratory, SciLifeLab,Limnologi,Uppsala University(Swepub:uu)stber839 (författare)
Dopson, MarkLinnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS(Swepub:lnu)madoaa (författare)
LinnéuniversitetetInstitutionen för biologi och miljö (BOM) (creator_code:org_t)

Ingår i:The ISME Journal: Springer Science and Business Media LLC10:5, s. 1192-12031751-73621751-7370

Av författaren/redakt...: Wu, Xiaofen; Holmfeldt, Karin; Hubalek, Valerie ...; Lundin, Daniel; Åström, Mats E.; Bertilsson, Stef ...; visa fler...; Dopson, Mark; visa färre...

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