L773:1432 0614 OR L773:0175 7598
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- Brodelius, PeterInstitute of Biotechnology, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Ziirich, Switzerland (author)
Permeabilization of Plant Cells for Release of Intracellularly Stored Products: Viability Studies
- Article/chapterEnglish1988
Publisher, publication year, extent ...
- 1988
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:lnu-598
- https://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-598URI
Supplementary language notes
- Language:English
- Summary in:English
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Notes
- The effects of various chemical substanceson the permeability of plasma membranesand tonoplasts of three suspension cultures (Catharanthusroseus, Thalictrum rugosum and Chenopodiumrubrum) have been studied. The permeabilityof the plasma membrane is monitoredby measuring the activity of the cytosolic enzymeisocitrate dehydrogenase and the permeability ofthe tonoplast is measured by determining the releaseof substances stored in the vacuoles (inorganicphosphate, berberine and betanin for thethree cell lines, respectively). The minimum concentrationrequired for quantitative release of vacuolarproducts have been established for five differentpermeabilization agents. Cell viability islost upon permeabilization except for treatmentof Catharanthus roseus with DMSO and Triton X-100.
Subject headings and genre
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- Biochemistry
- Biokemi
- Biochemistry
- Biokemi
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- Institute of Biotechnology, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Ziirich, Switzerland (creator_code:org_t)
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- In:Applied Microbiology and Biotechnology27, s. 561-5660175-75981432-0614
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