Inhibition of complement activation on a model biomaterial surface by streptococcal M protein-derived peptides

Engberg, Anna E.,1982-Högskolan i Kalmar,Naturvetenskapliga institutionen (författare)

The aim of this study was to evaluate a new approach to inhibit complement activation triggered by biomaterial surfaces in contact with blood. In order to inhibit complement activation initiated by the classical pathway (CP), we used streptococcal M protein-derived peptides that specifically bind human C4BP, an inhibitor of the CP. The peptides were used to coat polystyrene microtiter wells which served as a model biomaterial. The ability of coated peptides to bind C4BP and to attenuate complement activation via the CP (monitored as generation of fluid-phase C3a and binding of fragments of C3 and C4 to the surface) was investigated using diluted normal human serum, where complement activation by the AP is minimal, as well as serum from a patient lacking alternative pathway activation. Complement activation (all parameters) was significantly decreased in serum incubated in well surfaces coated with peptides. Total inhibition of complement activation was obtained at peptide coating concentrations as low as 1-5 mu g/mL. Successful use of Streptococcus-derived peptides shows that it is feasible to control complement activation at a model biomaterial surface by capturing autologous complement regulatory molecules from plasma. (C) 2009 Elsevier Ltd. All rights reserved.

NATURVETENSKAP Biologi Immunologi hsv//swe
NATURAL SCIENCES Biological Sciences Immunology hsv//eng
TEKNIK OCH TEKNOLOGIER Industriell bioteknik Bioteknisk apparatteknik hsv//swe
ENGINEERING AND TECHNOLOGY Industrial Biotechnology Bioengineering Equipment hsv//eng
Blood compatibility
Complement
C4b-binding protein (C4BP)
In vitro test
Regulator of complement activation (RCA)
Streptococcal M proteins
Immunologi
Immunology
test
In vitro
C4b-binding protein (C4BP)
Blood compatibility
Complement
Regulator of complement activation (RCA)
Streptococcal M proteins
MEDICINE

Sandholm, KerstinHögskolan i Kalmar,Naturvetenskapliga institutionen(Swepub:lnu)jsake (författare)
Bexborn, FredrikHögskolan i Kalmar,Naturvetenskapliga institutionen(Swepub:lnu)frbeaa (författare)
Persson, Jenny JLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)mmb-jpe (författare)
Nilsson, BoUppsala universitet,Enheten för klinisk immunologi(Swepub:uu)bonils (författare)
Lindahl, GunnarLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)mmb-gli (författare)
Nilsson Ekdahl, KristinaUppsala universitet,Högskolan i Kalmar,Naturvetenskapliga institutionen,Uppsala University,Enheten för klinisk immunologi(Swepub:uu)krisnil (författare)
Högskolan i KalmarNaturvetenskapliga institutionen (creator_code:org_t)

Av författaren/redakt...: Engberg, Anna E. ...; Sandholm, Kersti ...; Bexborn, Fredrik; Persson, Jenny J; Nilsson, Bo; Lindahl, Gunnar; visa fler...; Nilsson Ekdahl, ...; visa färre...

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