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Search: id:"swepub:oai:DiVA.org:lnu-73796" > Novel ATP-cone-driv...

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  • Rozman Grinberg, InnaStockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University (author)

Novel ATP-cone-driven allosteric regulation of ribonucleotide reductase via the radical-generating subunit

  • Article/chapterEnglish2018

Publisher, publication year, extent ...

  • ELIFE SCIENCES PUBLICATIONS LTD,2018
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:lnu-73796
  • https://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-73796URI
  • https://doi.org/10.7554/eLife.31529DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-144937URI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-153793URI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-345724URI
  • https://lup.lub.lu.se/record/1996e8d2-e4b2-4d0b-9e29-99584aa843a1URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Ribonucleotide reductases (RNRs) are key enzymes in DNA metabolism, with allosteric mechanisms controlling substrate specificity and overall activity. In RNRs, the activity master-switch, the ATP-cone, has been found exclusively in the catalytic subunit. In two class I RNR subclasses whose catalytic subunit lacks the ATP-cone, we discovered ATP-cones in the radical-generating subunit. The ATP-cone in the Leeuwenhoekiella blandensis radical-generating subunit regulates activity via quaternary structure induced by binding of nucleotides. ATP induces enzymatically competent dimers, whereas dATP induces non-productive tetramers, resulting in different holoenzymes. The tetramer forms by interactions between ATP-cones, shown by a 2.45 A crystal structure. We also present evidence for an (MnMnIV)-Mn-III metal center. In summary, lack of an ATP-cone domain in the catalytic subunit was compensated by transfer of the domain to the radical-generating subunit. To our knowledge, this represents the first observation of transfer of an allosteric domain between components of the same enzyme complex.

Subject headings and genre

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  • Lundin, Daniel,1965-Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University(Swepub:su)dlund (author)
  • Hasan, MahmudulStockholm University,Lunds universitet,Stockholms universitet,Institutionen för biokemi och biofysik,Lund University, Sweden,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.;Lund Univ, Dept Biochem & Struct Biol, Lund, Sweden.,Stockholm University ; Lund University,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)med-mmh (author)
  • Crona, MikaelSwedish Ophan Biovitrum AB, Stockholm, Sweden. (author)
  • Rao Jonna, VenkateswaraUmeå universitet,Institutionen för medicinsk kemi och biofysik,Umea Univ, Dept Med Biochem & Biophys, Umea, Sweden.,Umeå University(Swepub:umu)vera0003 (author)
  • Loderer, ChrishtophStockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University(Swepub:su)clode (author)
  • Sahlin, MargaretaStockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University(Swepub:su)msahl (author)
  • Markova, NataliaMalvern Instruments Inc, Malvern, Sweden.(Swepub:lu)extLU-753 (author)
  • Borovok, IlyaTel Aviv University, Israel,Tel Aviv Univ, Dept Mol Microbiol & Biotechnol, Tel Aviv, Israel. (author)
  • Berggren, GustavUppsala universitet,Molekylär biomimetik,Uppsala University(Swepub:uu)guber255 (author)
  • Hofer, AndersUmeå universitet,Institutionen för medicinsk kemi och biofysik,Umea Univ, Dept Med Biochem & Biophys, Umea, Sweden.,Umeå University(Swepub:umu)anho0002 (author)
  • Logan, Derek T.Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)mbfys-do (author)
  • Sjöberg, Britt-MarieStockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University(Swepub:su)bsjob (author)
  • Stockholms universitetInstitutionen för biokemi och biofysik (creator_code:org_t)

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