Search: id:"swepub:oai:DiVA.org:lnu-73796" >
Novel ATP-cone-driv...
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Rozman Grinberg, InnaStockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University
(author)
Novel ATP-cone-driven allosteric regulation of ribonucleotide reductase via the radical-generating subunit
- Article/chapterEnglish2018
Publisher, publication year, extent ...
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ELIFE SCIENCES PUBLICATIONS LTD,2018
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:lnu-73796
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https://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-73796URI
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https://doi.org/10.7554/eLife.31529DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-144937URI
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-153793URI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-345724URI
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https://lup.lub.lu.se/record/1996e8d2-e4b2-4d0b-9e29-99584aa843a1URI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Ribonucleotide reductases (RNRs) are key enzymes in DNA metabolism, with allosteric mechanisms controlling substrate specificity and overall activity. In RNRs, the activity master-switch, the ATP-cone, has been found exclusively in the catalytic subunit. In two class I RNR subclasses whose catalytic subunit lacks the ATP-cone, we discovered ATP-cones in the radical-generating subunit. The ATP-cone in the Leeuwenhoekiella blandensis radical-generating subunit regulates activity via quaternary structure induced by binding of nucleotides. ATP induces enzymatically competent dimers, whereas dATP induces non-productive tetramers, resulting in different holoenzymes. The tetramer forms by interactions between ATP-cones, shown by a 2.45 A crystal structure. We also present evidence for an (MnMnIV)-Mn-III metal center. In summary, lack of an ATP-cone domain in the catalytic subunit was compensated by transfer of the domain to the radical-generating subunit. To our knowledge, this represents the first observation of transfer of an allosteric domain between components of the same enzyme complex.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Lundin, Daniel,1965-Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University(Swepub:su)dlund
(author)
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Hasan, MahmudulStockholm University,Lunds universitet,Stockholms universitet,Institutionen för biokemi och biofysik,Lund University, Sweden,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.;Lund Univ, Dept Biochem & Struct Biol, Lund, Sweden.,Stockholm University ; Lund University,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)med-mmh
(author)
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Crona, MikaelSwedish Ophan Biovitrum AB, Stockholm, Sweden.
(author)
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Rao Jonna, VenkateswaraUmeå universitet,Institutionen för medicinsk kemi och biofysik,Umea Univ, Dept Med Biochem & Biophys, Umea, Sweden.,Umeå University(Swepub:umu)vera0003
(author)
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Loderer, ChrishtophStockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University(Swepub:su)clode
(author)
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Sahlin, MargaretaStockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University(Swepub:su)msahl
(author)
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Markova, NataliaMalvern Instruments Inc, Malvern, Sweden.(Swepub:lu)extLU-753
(author)
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Borovok, IlyaTel Aviv University, Israel,Tel Aviv Univ, Dept Mol Microbiol & Biotechnol, Tel Aviv, Israel.
(author)
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Berggren, GustavUppsala universitet,Molekylär biomimetik,Uppsala University(Swepub:uu)guber255
(author)
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Hofer, AndersUmeå universitet,Institutionen för medicinsk kemi och biofysik,Umea Univ, Dept Med Biochem & Biophys, Umea, Sweden.,Umeå University(Swepub:umu)anho0002
(author)
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Logan, Derek T.Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)mbfys-do
(author)
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Sjöberg, Britt-MarieStockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.,Stockholm University(Swepub:su)bsjob
(author)
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Stockholms universitetInstitutionen för biokemi och biofysik
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