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Genomes from uncult...
Genomes from uncultivated prokaryotes : a comparison of metagenome-assembled and single-amplified genomes
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- Alneberg, Johannes (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab,KTH Royal Institute of Technology, Sweden,KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden
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- Karlsson, Christofer M. G. (författare)
- Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Linnaeus Univ, Ctr Ecol & Evolut Microbial Model Syst, EEMiS, Kalmar, Sweden.
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- Divne, Anna-Maria (författare)
- Uppsala universitet,Molekylär evolution,Science for Life Laboratory, SciLifeLab
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- Bergin, Claudia (författare)
- Uppsala universitet,Molekylär evolution,Science for Life Laboratory, SciLifeLab
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- Homa, Felix (författare)
- Uppsala universitet,Molekylär evolution,Science for Life Laboratory, SciLifeLab
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- Lindh, Markus V., 1985- (författare)
- Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Lund University, Sweden,Linnaeus Univ, Ctr Ecol & Evolut Microbial Model Syst, EEMiS, Kalmar, Sweden.;Lund Univ, Dept Biol, Lund, Sweden.
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- Hugerth, Luisa, 1987- (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab,KTH Royal Institute of Technology, Sweden;Karolinska Institutet, Sweden,KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden;Karolinska Inst, Ctr Translat Microbiome Res, Dept Mol Tumour & Cell Biol, Sci Life Lab, Solna, Sweden
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- Ettema, Thijs J. G. (författare)
- Uppsala universitet,Molekylär evolution,Science for Life Laboratory, SciLifeLab
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- Bertilsson, Stefan (författare)
- Uppsala universitet,Limnologi,Science for Life Laboratory, SciLifeLab
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- Andersson, Anders F. (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab,KTH Royal Institute of Technology, Sweden,KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden
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- Pinhassi, Jarone (författare)
- Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Linnaeus Univ, Ctr Ecol & Evolut Microbial Model Syst, EEMiS, Kalmar, Sweden.
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(creator_code:org_t)
- 2018-09-28
- 2018
- Engelska.
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Ingår i: Microbiome. - : BioMed Central. - 2049-2618. ; 6
- Relaterad länk:
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https://doi.org/10.1...
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https://microbiomejo...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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http://kipublication...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Background: Prokaryotes dominate the biosphere and regulate biogeochemical processes essential to all life. Yet, our knowledge about their biology is for the most part limited to the minority that has been successfully cultured. Molecular techniques now allow for obtaining genome sequences of uncultivated prokaryotic taxa, facilitating in-depth analyses that may ultimately improve our understanding of these key organisms. Results: We compared results from two culture-independent strategies for recovering bacterial genomes: single-amplified genomes and metagenome-assembled genomes. Single-amplified genomes were obtained from samples collected at an offshore station in the Baltic Sea Proper and compared to previously obtained metagenome-assembled genomes from a time series at the same station. Among 16 single-amplified genomes analyzed, seven were found to match metagenome-assembled genomes, affiliated with a diverse set of taxa. Notably, genome pairs between the two approaches were nearly identical (average 99.51% sequence identity; range 98.77-99.84%) across overlapping regions (30-80% of each genome). Within matching pairs, the single-amplified genomes were consistently smaller and less complete, whereas the genetic functional profiles were maintained. For the metagenome-assembled genomes, only on average 3.6% of the bases were estimated to be missing from the genomes due to wrongly binned contigs. Conclusions: The strong agreement between the single-amplified and metagenome-assembled genomes emphasizes that both methods generate accurate genome information from uncultivated bacteria. Importantly, this implies that the research questions and the available resources are allowed to determine the selection of genomics approach for microbiome studies.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Nyckelord
- Single-amplified genomes
- Metagenome-assembled genomes
- Metagenomics
- Binning
- Single-cell genomics
- Mikrobiologi
- Microbiology
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- art (ämneskategori)
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Alneberg, Johann ...
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Karlsson, Christ ...
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Divne, Anna-Mari ...
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Bergin, Claudia
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Homa, Felix
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Lindh, Markus V. ...
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Hugerth, Luisa, ...
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Ettema, Thijs J. ...
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Bertilsson, Stef ...
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Andersson, Ander ...
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Pinhassi, Jarone
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- NATURVETENSKAP
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och Biologi
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och Mikrobiologi
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NATURVETENSKAP
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och Biologi
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Microbiome
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Linnéuniversitetet
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Kungliga Tekniska Högskolan
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Karolinska Institutet
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Uppsala universitet