Insights into cyanophage-mediated dynamics of nodularin and other non-ribosomal peptides in Nodularia spumigena

• The effect of cyanophage infection and lysis on the dynamics of the hepatotoxin nodularin (NOD) and other nonribosomal peptides (NRPs) produced by cyanobacteria is poorly understood. In this study, changes in concentration of NOD and other NRPs during cyanophage infection of the filamentous cyanobacteria Nodularia spumigena were assessed using incubation experiments. Viral infection and lysis were associated with a significant reduction (93% at the 96 h post infection) of N. spumigena cell density. While no correlation between N. spumigena abundance and total concentration of NOD (ng mL(-1)) within the infected cells was observed, cellular NOD quota (ng cell(-1)) gradually increased in the remaining cyanophage resistant N. spumigena subpopulation. Lysis of N. spurnigena cells resulted in a substantial increase (>57 times) of dissolved NOD concentration in the culture medium. The relative concentration of other cyclic (anabaenopeptins) and linear (aeruginosins, spumigins) NRPs produced by N. spumigena also increased in response to cyanophage addition. This study highlights the importance of cyanophage infection on the population toxicity of filamentous cyanobacteria and demonstrates a significant contribution of virus-mediated cell lysis on the conversion of NOD from the particulate to dissolved phase.

Ämnesord

NATURVETENSKAP  -- Biologi -- Ekologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Ecology (hsv//eng)

Nyckelord

Aquatic viruses

Baltic Sea

Cyanotoxins

Filamentous cyanobacteria

virus-bacterium interactions

Akvatisk ekologi

Aquatic Ecology

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)