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QCM sensing of mult...
QCM sensing of multivalent interactions between lectins and well-defined glycosylated nanoplatforms
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- Abellan-Flos, Marta (författare)
- Univ Namur, Dept Chim, Lab Chim Bioorgan, Rue Bruxelles 61, B-5000 Namur, Belgium.;PSL Univ, CNRS, ESPCI Paris, Mol Macromol Chem & Mat, 10 Rue Vauquelin, F-75005 Paris, France.
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- Timmer, Brian J. J. (författare)
- KTH,Kemi,KTH Royal Institute of Technology, Sweden
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- Altun, Samuel (författare)
- Attana AB, Bjornnasvagen 21, S-11419 Stockholm, Sweden.
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- Aastrup, Teodor (författare)
- Attana AB, Bjornnasvagen 21, S-11419 Stockholm, Sweden.,Linnaeus Ctr Biomat Chem, BMC;BBCL
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- Vincent, Stephane P. (författare)
- Univ Namur, Dept Chim, Lab Chim Bioorgan, Rue Bruxelles 61, B-5000 Namur, Belgium.
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- Ramström, Olof (författare)
- KTH,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),KTH Royal Institute of Technology, Sweden;Univ Massachusetts, USA,Linnaeus Ctr Biomat Chem, BMC;BBCL,Kemi,Univ Massachusetts, Dept Chem, One Univ Ave, Lowell, MA 01854 USA.;Linnaeus Univ, Dept Chem & Biomed Sci, SE-39182 Kalmar, Sweden.
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Univ Namur, Dept Chim, Lab Chim Bioorgan, Rue Bruxelles 61, B-5000 Namur, Belgium;PSL Univ, CNRS, ESPCI Paris, Mol Macromol Chem & Mat, 10 Rue Vauquelin, F-75005 Paris, France. Kemi (creator_code:org_t)
- Elsevier, 2019
- 2019
- Engelska.
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Ingår i: Biosensors & bioelectronics. - : Elsevier. - 0956-5663 .- 1873-4235. ; 139
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Quartz crystal microbalance (QCM) methodology has been adopted to unravel important factors contributing to the "cluster glycoside effect" observed in carbohydrate-lectin interactions. Well-defined, glycosylated nanostructures of precise sizes, geometries and functionalization patterns were designed and synthesized, and applied to analysis of the interaction kinetics and thermodynamics with immobilized lectins. The nanostructures were based on Borromean rings, dodecaamine cages, and fullerenes, each of which carrying a defined number of carbohydrate ligands at precise locations. The synthesis of the Borromeates and dodecaamine cages was easily adjustable due to the modular assembly of the structures, resulting in variations in presentation mode. The binding properties of the glycosylated nanoplatforms were evaluated using flow-through QCM technology, as well as hemagglutination inhibition assays, and compared with dodecaglycosylated fullerenes and a monovalent reference. With the QCM setup, the association and dissociation rate constants and the associated equilibrium constants of the interactions could be estimated, and the results used to delineate the multivalency effects of the lectin-nanostructure interactions.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Nyckelord
- QCM
- Carbohydrates
- Lectins
- Multivalency
- Nanoplatforms
- Kemi
- Chemistry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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