SwePub
Sök i LIBRIS databas

  Extended search

id:"swepub:oai:DiVA.org:lnu-89282"
 

Search: id:"swepub:oai:DiVA.org:lnu-89282" > Genomic and Seasona...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Nilsson, EmelieLinnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS (author)

Genomic and Seasonal Variations among Aquatic Phages Infecting the Baltic Sea Gammaproteobacterium Rheinheimera sp. Strain BAL341

  • Article/chapterEnglish2019

Publisher, publication year, extent ...

  • American Society for Microbiology,2019
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:lnu-89282
  • https://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-89282URI
  • https://doi.org/10.1128/AEM.01003-19DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-163365URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Knowledge in aquatic virology has been greatly improved by culture-independent methods, yet there is still a critical need for isolating novel phages to identify the large proportion of "unknowns" that dominate metagenomes and for detailed analyses of phage-host interactions. Here, 54 phages infecting Rheinheimem sp. strain BAL341 (Gammaproteobacteria) were isolated from Baltic Sea seawater and characterized through genome content analysis and comparative genomics. The phages showed a myovirus-like morphology and belonged to a novel genus, for which we propose the name Barbavirus. All phages had similar genome sizes and numbers of genes (80 to 84 kb; 134 to 145 genes), and based on average nucleotide identity and genome BLAST distance phylogeny, the phages were divided into five species. The phages possessed several genes involved in metabolic processes and host signaling, such as genes encoding ribonucleotide reductase and thymidylate synthase, phoH, and rnazG. One species had additional metabolic genes involved in pyridine nucleotide salvage, possibly providing a fitness advantage by further increasing the phages' replication efficiency. Recruitment of viral metagenomic reads (25 Baltic Sea viral metagenomes from 2012 to 2015) to the phage genomes showed pronounced seasonal variations, with increased relative abundances of barba phages in August and September synchronized with peaks in host abundances, as shown by 16S rRNA gene amplicon sequencing. Overall, this study provides detailed information regarding genetic diversity, phage-host interactions, and temporal dynamics of an ecologically important aquatic phage-host system. IMPORTANCE Phages are important in aquatic ecosystems as they influence their microbial hosts through lysis, gene transfer, transcriptional regulation, and expression of phage metabolic genes. Still, there is limited knowledge of how phages interact with their hosts, especially at fine scales. Here, a Rheinheimera phage-host system constituting highly similar phages infecting one host strain is presented. This relatively limited diversity has previously been seen only when smaller numbers of phages have been isolated and points toward ecological constraints affecting the Rheinheimera phage diversity. The variation of metabolic genes among the species points toward various fitness advantages, opening up possibilities for future hypothesis testing. Phage-host dynamics monitored over several years point toward recurring "kill-the-winner" oscillations and an ecological niche fulfilled by this system in the Baltic Sea. Identifying and quantifying ecological dynamics of such phage-host model systems in situ allow us to understand and study the influence of phages on aquatic ecosystems.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Li, K.Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS (author)
  • Fridlund, JimmyLinnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS(Swepub:lnu)jifraa (author)
  • Šulčius, SigitasLinnéuniversitetet,Institutionen för biologi och miljö (BOM),Nat Res Ctr, Lithuania,Ctr Ecol & Evolut Microbial Model Syst EEMiS (author)
  • Bunse, CarinaLinnéuniversitetet,Institutionen för biologi och miljö (BOM),Helmholtz Zentrum Polar & Meeresforsch, Germany;Carl von Ossietzky Univ Oldenburg, Germany,Ctr Ecol & Evolut Microbial Model Syst EEMiS,EcoChange(Swepub:lnu)cabuaa (author)
  • Karlsson, Christofer M. G.Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS(Swepub:lnu)chkaab (author)
  • Lindh, Markus V.,1985-Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Swedish Meteorological and Hydrological Institute, Sweden,Ctr Ecol & Evolut Microbial Model Syst EEMiS,EcoChange(Swepub:lnu)flima (author)
  • Lundin, Daniel,1965-Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS(Swepub:lnu)daluab (author)
  • Pinhassi, JaroneLinnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS,EcoChange(Swepub:lnu)ypija (author)
  • Holmfeldt, Karin,1980-Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Ctr Ecol & Evolut Microbial Model Syst EEMiS(Swepub:lnu)mhoka (author)
  • LinnéuniversitetetInstitutionen för biologi och miljö (BOM) (creator_code:org_t)

Related titles

  • In:Applied and Environmental Microbiology: American Society for Microbiology85:18, s. 1-190099-22401098-5336

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view