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Measurement of Asco...
Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
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- Aguilera, Anabella (författare)
- Fdn Invest Biol Aplicadas CIB FIBA, Argentina
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- Steelheart, Charlotte (författare)
- Univ Nacl La Plata, Argentina
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- Alegre, Matias (författare)
- Univ Nacl La Plata, Argentina
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- Berdun, Federico (författare)
- Fdn Invest Biol Aplicadas CIB FIBA, Argentina
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- Salerno, Graciela (författare)
- Fdn Invest Biol Aplicadas CIB FIBA, Argentina
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- Bartoli, Carlos (författare)
- Univ Nacl La Plata, Argentina
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- Pagnussat, Gabriela (författare)
- Univ Nacl Mar del Plata, Argentina
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- Victoria Martin, Maria (författare)
- Fdn Invest Biol Aplicadas CIB FIBA, Argentina
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(creator_code:org_t)
- Bio-protocol, 2020
- 2020
- Engelska.
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Ingår i: Bio-protocol. - : Bio-protocol. - 2331-8325. ; 10:20, s. 1-7
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.2...
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Abstract
Ämnesord
Stäng
- Ascorbic acid (AsA) and gluthathione (GSH) are two key components of the antioxidant machinery of eukaryotic and prokaryotic cells. The cyanobacterium Synechocystis sp. PCC 6803 presents both compounds in different concentrations (AsA, 20-100 mu M and GSH, 2-5 mM). Therefore, it is important to have precise and sensitive methods to determine the redox status in the cell and to detect variations in this antioxidants. In this protocol, we describe an improved method to estimate the content of both antioxidants (in their reduced and oxidized forms) from the same sample obtained from liquid cultures of Synechocystis sp. PCC 6803.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- Cyanobacteria
- Antioxidants
- Glutathione
- Ascorbic acid
- Oxidative stress
- Mikrobiologi
- Microbiology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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