Sökning: WFRF:(Kurt Widhalm) >
N-Acetylcysteine Am...
N-Acetylcysteine Amide Exerts Possible Neuroprotective Effects in Newborn Pigs after Perinatal Asphyxia
-
Benterud, T (författare)
-
Ystgaard, MB (författare)
-
Manueldas, S (författare)
-
visa fler...
-
Pankratov, L (författare)
-
Alfaro-Cervello, C (författare)
-
Florholnnen, G (författare)
-
Ahmed, MS (författare)
-
Sandvik, L (författare)
-
- Norgren, S (författare)
- Karolinska Institutet
-
Bjoras, M (författare)
-
Baumbusch, LO (författare)
-
Solberg, R (författare)
-
Saugstad, OD (författare)
-
visa färre...
-
(creator_code:org_t)
- 2016-08-06
- 2017
- Engelska.
-
Ingår i: Neonatology. - : S. Karger AG. - 1661-7819 .- 1661-7800. ; 111:1, s. 12-21
- Relaterad länk:
-
http://kipublication...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- <b><i>Background:</i></b> Perinatal asphyxia and ensuing reoxygenation change the antioxidant capacity of cells and organs. <b><i>Objectives:</i></b> To analyze the neuroprotective effect of the antioxidant N-acetylcysteine amide (NACA) after perinatal hypoxia-reoxygenation with an emphasis on proinflammatory cytokines and the transcription factor NF-κB in the prefrontal cortex of neonatal pigs. <b><i>Methods:</i></b> Twenty-nine newborn pigs, aged 12-36 h, were subjected to global hypoxia and hypercapnia. One sham-operated group (n = 5) and 2 experimental groups (n = 12) were exposed to 8% oxygen, until the base excess was -20 mmol/l or the mean arterial blood pressure fell to <20 mm Hg (asphyxia with NACA or saline). The pigs were observed for 9.5 h after hypoxia. Samples of prefrontal cortex and plasma were analyzed. <b><i>Results:</i></b> Cortex: there was no significant difference in mRNA expression between the intervention groups regarding IL-1β, IL6, TNFα, MMP2, MMP9 or IL18. Pigs exposed to hypoxia-reoxygenation and treatment with NACA (NACA-pigs) had a significantly lower protein concentration of IL-1β than pigs treated with saline (placebo controls), i.e. 8.8 ± 3.9 versus 16.8 ± 10.5 pg/mg protein (p = 0.02). The activation of the transcription factor NF-κB (measured as the fold-change of phosphorylated p65<sup>Ser 536</sup>), was reduced in the NACA-pigs when compared to the placebo controls (5.2 ± 4.3 vs. 16.0 ± 13.5; p = 0.02). No difference between the intervention groups regarding brain histopathology or in the levels of 8-oxoguanine measured in the prefrontal cortex were observed. Plasma: the NACA-pigs had a stronger reduction of TNFα in the first 30 min following asphyxia compared with the placebo controls, i.e. 36 (30-44) versus 24 (14-32)% (p = 0.01). <b><i>Conclusion:</i></b> The reduced levels of the pivotal inflammatory markers IL-1β and TNFα and the transcription factor NF-κB may indicate that NACA has possible neuroprotective effects after perinatal asphyxia.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Benterud, T
-
Ystgaard, MB
-
Manueldas, S
-
Pankratov, L
-
Alfaro-Cervello, ...
-
Florholnnen, G
-
visa fler...
-
Ahmed, MS
-
Sandvik, L
-
Norgren, S
-
Bjoras, M
-
Baumbusch, LO
-
Solberg, R
-
Saugstad, OD
-
visa färre...
- Artiklar i publikationen
-
Neonatology
- Av lärosätet
-
Karolinska Institutet